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CD32B antibody [C2C3], C-term

Anti-CD32B antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100262
Anti-CD32B antibody [C2C3], C-term used in Western Blot (WB). GTX100262

Cat No. GTX100262

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, FACS
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

THP-1
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CD32B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fc fragment of IgG receptor IIb,CD32,CD32B,FCG2,FCGR2,FCGR2C,FcRII-c,IGFR2

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
Anti-CD32B antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100262

GTX100262 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using CD32B(GTX100262) antibody at 1:500 dilution.

Anti-CD32B antibody [C2C3], C-term used in Western Blot (WB). GTX100262

GTX100262 WB Image

Sample (30 µg of whole cell lysate)
A: THP-1
10% SDS PAGE
GTX100262 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169