APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody was raised against human CD32B Intracellular domain.
Predict Reactivity
Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of human CD32B. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Fc fragment of IgG receptor IIb , CD32 , CD32B , FCG2 , FCGR2 , FCGR2C , FcRII-c , IGFR2
Cellular Localization
Cell membrane; Single-pass type I membrane protein
Background
The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Database
Research Area
DATA IMAGES
![Anti-CD32B antibody [HL1948] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637785](/templates/default/js/lightbox/images/loading.gif "Anti-CD32B antibody [HL1948] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637785")
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GTX637785 ICC/IF Image
CD32B antibody [HL1948] detects CD32B protein at cell membrane by immunofluorescent analysis.Sample: U937 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD32B stained by CD32B antibody [HL1948] (GTX637785) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637785 IHC-P Image
CD32B antibody [HL1948] detects CD32B protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.CD32B stained by CD32B antibody [HL1948] (GTX637785) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637785 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD32B antibody [HL1948] (GTX637785) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX637785 ICC/IF Image
CD32B antibody [HL1948] detects CD32B protein by immunofluorescent analysis.Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD32B stained by CD32B antibody [HL1948] (GTX637785) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for CD32B antibody [HL1948] (GTX637785). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD32B antibody [HL1948] (GTX637785). Be the first to share your experience with this product.
![Anti-CD32B antibody [HL1948] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637785](/upload/website/prouct_img/small/GTX637785/GTX637785_T-44851_20230106_ICC_IF_23013122_356.webp "Anti-CD32B antibody [HL1948] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637785")
![Anti-CD32B antibody [HL1948] used in IHC (Paraffin sections) (IHC-P). GTX637785](/upload/website/prouct_img/small/GTX637785/GTX637785_T-44851_20221223_IHC-P_R_22122722_385.webp "Anti-CD32B antibody [HL1948] used in IHC (Paraffin sections) (IHC-P). GTX637785")
![Anti-CD32B antibody [HL1948] used in Western Blot (WB). GTX637785](/upload/website/prouct_img/small/GTX637785/GTX637785_44900_20221223_WB_TPM_watermark_22122722_110.webp "Anti-CD32B antibody [HL1948] used in Western Blot (WB). GTX637785")
![Anti-CD32B antibody [HL1948] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637785](/upload/website/prouct_img/small/GTX637785/GTX637785_44900_20221230_ICC_IF_23010219_166.webp "Anti-CD32B antibody [HL1948] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637785")
![Anti-CD32B antibody [HL1948] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637785](/upload/website/prouct_img/normal/GTX637785/GTX637785_T-44851_20230106_ICC_IF_23013122_356.webp "Anti-CD32B antibody [HL1948] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637785")
![Anti-CD32B antibody [HL1948] used in IHC (Paraffin sections) (IHC-P). GTX637785](/upload/website/prouct_img/normal/GTX637785/GTX637785_T-44851_20221223_IHC-P_R_22122722_385.webp "Anti-CD32B antibody [HL1948] used in IHC (Paraffin sections) (IHC-P). GTX637785")
![Anti-CD32B antibody [HL1948] used in Western Blot (WB). GTX637785](/upload/website/prouct_img/normal/GTX637785/GTX637785_44900_20221223_WB_TPM_watermark_22122722_110.webp "Anti-CD32B antibody [HL1948] used in Western Blot (WB). GTX637785")
![Anti-CD32B antibody [HL1948] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637785](/upload/website/prouct_img/normal/GTX637785/GTX637785_44900_20221230_ICC_IF_23010219_166.webp "Anti-CD32B antibody [HL1948] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637785")