Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD32B antibody [HL1950] (GTX637787) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

CD32B antibody [HL1950] detects CD32B protein at cell membrane by immunofluorescent analysis.
Sample: U937 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD32B stained by CD32B antibody [HL1950] (GTX637787) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).