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CD34 antibody [HPCA1/1171]

Anti-CD34 antibody [HPCA1/1171] used in IHC (Paraffin sections) (IHC-P). GTX34500
Anti-CD34 antibody [HPCA1/1171] used in IHC (Paraffin sections) (IHC-P). GTX34500
Anti-CD34 antibody [HPCA1/1171] used in Flow cytometry (FACS). GTX34500

Cat No. GTX34500

Host Mouse
Clonality Monoclonal
Clone Name HPCA1/1171
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 2-4ug/ml for 30 minutes at RT
FACS Assay dependent

Note :

IHC-P
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but shows low specificity.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full-length human HPCA1 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd34 Molecule,Cd34

Background

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES
Anti-CD34 antibody [HPCA1/1171] used in IHC (Paraffin sections) (IHC-P). GTX34500

GTX34500 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)

Anti-CD34 antibody [HPCA1/1171] used in IHC (Paraffin sections) (IHC-P). GTX34500

GTX34500 IHC-P Image

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Monoclonal Antibody (HPCA1/1171)

Anti-CD34 antibody [HPCA1/1171] used in Flow cytometry (FACS). GTX34500

GTX34500 FACS Image

Flow Cytometry of KG-1 cells using CD34 Monoclonal Antibody (HPCA1/1171) (red) & isotype control (green).

Package List Price ($)
$ 329