GeneTex
United States (US)

CD34 antibody [HPCA1/763]

GTX34499_20170712_IHC-P_1_18121410_678.jpg
GTX34499_20170712_IHC-P_2_18121410_816.jpg
GTX34499_20170712_IHC-P_18121410_812.jpg

Cat No. GTX34499

Host Mouse
Clonality Monoclonal
Clone Name HPCA1/763
Isotype IgG1
Application IHC-P
Reactivity Human
APPLICATION

Application Note

IHC-P:0.25-0.5ug/ml for 30 minutes at RT.

Calculated MW

41 kDa. ( Note )

Specificity/Sensitivity

This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but with a lower specificity.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full-length human HPCA1 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd34 Molecule,Cd34

Background

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES
GTX34499_20170712_IHC-P_1_18121410_678.jpg

GTX34499 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/763)

GTX34499_20170712_IHC-P_2_18121410_816.jpg

GTX34499 IHC-P Image

Formalin-fixed, paraffin-embedded human Placenta stained with CD34 Monoclonal Antibody (HPCA1/763)

GTX34499_20170712_IHC-P_18121410_812.jpg

GTX34499 IHC-P Image

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Monoclonal Antibody (HPCA1/763)

Package List Price ($)
$ 329