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CD34 antibody [ICO-115]

Anti-CD34 antibody [ICO-115] used in Flow cytometry (FACS). GTX34496

Cat No. GTX34496

Host

Mouse

Clonality

Monoclonal

Clone Name

ICO-115

Isotype

IgG1

Application

ICC/IF, FACS, ELISA

Reactivity

Human, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
FACS 1-2μg/10⁶ cells
ELISA Assay dependent

Note :

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens).

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Blast cells of a chronic myeloid leukemia patient

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD34 , CD34 molecule

Cellular Localization

Membrane

Background

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-CD34 antibody [ICO-115] used in Flow cytometry (FACS). GTX34496

GTX34496 FACS Image

FACS analysis of KG-1 cells using GTX34496 CD34 antibody [ICO-115].
Red : Primary antibody
Green : Isotype control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339