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CD34 antibody [MEC14.7] (FITC)

Anti-CD34 antibody [MEC14.7] (FITC) used in Flow cytometry (FACS). GTX75411

Cat. No. GTX75411

Reference 40

Host

 Rat

Clonality

 Monoclonal

Clone Name

 MEC14.7

Isotype

 IgG2a

Application

 ICC/IF, FACS, IHC

Reactivity

 Human, Mouse, Rabbit
Package
100 μg ($399)
See all CD34 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
FACS Neat
IHC Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Product Note

This antibody recognizes a neuraminidase sensitive epitope.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

T-end.1, a pMT transformed endothelial cell line.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

RRID

AB_378417

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD34 antigen , AU040960

Cellular Localization

Membrane; Single-pass type I membrane protein

Database

Research Area

DATA IMAGES

Anti-CD34 antibody [MEC14.7] (FITC) used in Flow cytometry (FACS). GTX75411

GTX75411 FACS Image

FACS analysis of mouse bone marrow cells using GTX75411 CD34 antibody [MEC14.7] (FITC).

REFERENCE

Clone Reference

Fei Sun et al. Bioeng Transl Med. 2023;:e10534 Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one-step
Reactivity : Rabbit
Li K et al. Leukemia 2023; 37 (2):308-325 Single-cell analysis reveals the chemotherapy-induced cellular reprogramming and novel therapeutic targets in relapsed/refractory acute myeloid leukemia.
Xiao W et al. Sci Adv 2022; 8 (28):eabn1958 Recombinant DTβ4-inspired porous 3D vascular graft enhanced antithrombogenicity and recruited circulating CD93+/CD34+ cells for endothelialization.
Han C et al. Beijing Da Xue Xue Bao Yi Xue Ban 2022; 54 (6):1151-1157 [Biological characteristics of sheep peripheral blood mesenchymal stem cell].
Zhen Wang et al. Stem Cells Int 2021; 2021:9947187 Effect of Activated Platelet-Rich Plasma on Chondrogenic Differentiation of Rabbit Bone Marrow-Derived Mesenchymal Stem Cells.
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REVIEW

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Application Reference
Su LJ et al. Sci Rep 2017; 7:45607 Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs.
Reactivity : Human
Choi JH et al. Mol Cells 2014; 37 (6):487-96 Hypoxia-induced endothelial progenitor cell function is blunted in angiotensinogen knockout mice.
Application : FACS
Reactivity : Mouse
Urbani L et al. PLoS One 2012; 7 (11):e49860 Hypoxia increases mouse satellite cell clone proliferation maintaining both in vitro and in vivo heterogeneity and myogenic potential.
Application : ICC/IF
Jung SY et al. J Cell Biochem 2012; 113 (5):1478-87 Decursin inhibits vasculogenesis in early tumor progression by suppression of endothelial progenitor cell differentiation and function.
Application : FACS
Reactivity : Mouse
Peng YJ et al. J Biomed Mater Res A 2012; 100 (5):1126-35 the application of tissue engineering in reversing * c-induced ischemic conjunctiva.
Clone Reference
Fei Sun et al. Bioeng Transl Med. 2023;:e10534 Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one-step
Reactivity : Rabbit
Li K et al. Leukemia 2023; 37 (2):308-325 Single-cell analysis reveals the chemotherapy-induced cellular reprogramming and novel therapeutic targets in relapsed/refractory acute myeloid leukemia.
Xiao W et al. Sci Adv 2022; 8 (28):eabn1958 Recombinant DTβ4-inspired porous 3D vascular graft enhanced antithrombogenicity and recruited circulating CD93+/CD34+ cells for endothelialization.
Han C et al. Beijing Da Xue Xue Bao Yi Xue Ban 2022; 54 (6):1151-1157 [Biological characteristics of sheep peripheral blood mesenchymal stem cell].
Khorolskaya JI et al. Biomedicines 2021; 9 (9) Derivation and Characterization of EGFP-Labeled Rabbit Limbal Mesenchymal Stem Cells and Their Potential for Research in Regenerative Ophthalmology.
Xu H et al. Stem Cells Transl Med 2021; 10 (5):781-796 Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid-induced osteonecrosis of the femoral head.
Kikuchi S et al. Regen Ther 2021; 16:32-41 Development of a nasal mucosa-removal model for evaluating cell therapy.
Chopra H et al. Blood Cells Mol Dis 2021; 86:102487 CD133+CD34+ cells can give rise to EPCs: A comparative rabbit and human study.
Zhen Wang et al. Stem Cells Int 2021; 2021:9947187 Effect of Activated Platelet-Rich Plasma on Chondrogenic Differentiation of Rabbit Bone Marrow-Derived Mesenchymal Stem Cells.
Zhan JW et al. PLoS One 2020; 15 (6):e0234747 Constant compression decreases vascular bud and VEGFA expression in a rabbit vertebral endplate ex vivo culture model.
Nishi H et al. J Am Heart Assoc 2019; 8 (20):e014074 Biodegradable Flow Diverter for the Treatment of Intracranial Aneurysms: A Pilot Study Using a Rabbit Aneurysm Model.
Yeung TL et al. Clin Cancer Res 2019; Anticancer immunotherapy by MFAP5 blockade inhibits fibrosis and enhances chemosensitivity in ovarian and pancreatic cancer.
Niu L et al. J Biomed Mater Res B Appl Biomater 2018; (Epub) PLGA/PLCA casting and PLGA/PDPA electrospinning bilayer film for prevention of postoperative adhesion.
Chen B et al. J Cell Biochem 2018; (Epub) Characterization of the structure of rabbit anterior cruciate ligament and its stem/progenitor cells.
Koon YL et al. Sci Rep 2018; 8 (1):9519 Enhanced Delta-Notch Lateral Inhibition Model Incorporating Intracellular Notch Heterogeneity and Tension-Dependent Rate of Delta-Notch Binding that Reproduces Sprouting Angiogenesis Patterns.
Tiwary S et al. Sci Rep 2018; 8 (1):8267 Metastatic Brain Tumors Disrupt the Blood-Brain Barrier and Alter Lipid Metabolism by Inhibiting Expression of the Endothelial Cell Fatty Acid Transporter Mfsd2a.
Jia Z et al. J Transl Med 2018; 16 (1):123 Repair of articular cartilage defects with intra-articular injection of autologous rabbit synovial fluid-derived mesenchymal stem cells.
Lee HY et al. Oncotarget 2018; 9 (17):13193-13205 EIF3C-enhanced exosome secretion promotes angiogenesis and tumorigenesis of human hepatocellular carcinoma.
Okubo K et al. Nat Med 2018; (Epub) Macrophage extracellular trap formation promoted by platelet activation is a key mediator of rhabdomyolysis-induced acute kidney injury.
Kwiatkowski SC et al. PLoS One 2017; 12 (9):e0185065 Neuropilin-1 modulates TGFβ signaling to drive glioblastoma growth and recurrence after anti-angiogenic therapy.
Ma X et al. J Cell Sci 2017; Nonmuscle myosin II-B regulates epicardial integrity and epicardial derived mesenchymal cell maturation.
Jiang P et al. Exp Biol Med (Maywood) 2015; 240 (7):876-83 Phagocytosis of platelets enhances endothelial cell survival under serum deprivation.
Han MS et al. Quant Imaging Med Surg 2014; 4 (3):156-62 Lessons learned from imaging mouse ovarian tumors: the route of probe injection makes a difference.
Hirahashi J et al. Sci Rep 2014; 4:6406 Immunomodulation with eicosapentaenoic acid supports the treatment of autoimmune small-vessel vasculitis.
Yang J et al. J Res Med Sci 2014; 19 (2):112-6 Adipose-derived stem cells from the breast.
Lee TC et al. PLoS One 2014; 9 (11):e111390 Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells.
Schem C et al. BMC Cancer 2013; 13:32 Preclinical evaluation of * as a single agent in the prophylactic setting in a mouse model of bone metastases.
Uchibori R et al. Cancer Res 2013; 73 (1):364-72 NF-κB activity regulates mesenchymal stem cell accumulation at tumor sites.
Application : IHC
Kubo N et al. J Gastroenterol Hepatol 2012; 27 (1):165-72 Efficacy of adipose tissue-derived mesenchymal stem cells for fulminant hepatitis in mice induced by concanavalin A.
Tchaicha JH et al. Cancer Res 2011; 71 (20):6371-81 Glioblastoma angiogenesis and tumor cell invasiveness are differentially regulated by β8 integrin.
Tchaicha JH et al. Oncogene 2010; 29 (31):4460-72 A mosaic mouse model of astrocytoma identifies alphavbeta8 integrin as a negative regulator of tumor angiogenesis.
Spirli C et al. Gastroenterology 2010; 138 (1):360-371.e7 ERK1/2-dependent vascular endothelial growth factor signaling sustains cyst growth in polycystin-2 defective mice.
Truman JP et al. PLoS One 2010; 5 (8):e12310 Endothelial membrane remodeling is obligate for anti-angiogenic radiosensitization during tumor radiosurgery.
Application : IHC
Reactivity : Mouse
Hirahashi J et al. Circulation 2009; 120 (13):1255-65 Mac-1 (CD11b/CD18) links inflammation and thrombosis after glomerular injury.
Trempus CS et al. J Invest Dermatol 2003; 120 (4):501-11 Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399