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CD34 antibody [MEC14.7] (FITC)

Anti-CD34 antibody [MEC14.7] (FITC) used in Flow cytometry (FACS). GTX75412

Cat. No. GTX75412

Reference 40

Host

 Rat

Clonality

 Monoclonal

Clone Name

 MEC14.7

Isotype

 IgG2a

Application

 ICC/IF, FACS, IHC

Reactivity

 Mouse, Rabbit
Package
100 μg ($339)
See all CD34 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
FACS 1/5-1/10
IHC Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Product Note

This antibody recognizes a neuraminidase sensitive epitope.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

T-end.1, a pMT transformed endothelial cell line.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

RRID

AB_378418

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD34 antigen , AU040960

Cellular Localization

Membrane; Single-pass type I membrane protein

Database

Research Area

DATA IMAGES

Anti-CD34 antibody [MEC14.7] (FITC) used in Flow cytometry (FACS). GTX75412

GTX75412 FACS Image

FACS analysis of mouse bone marrow cells using GTX75412 CD34 antibody [MEC14.7] (FITC).

REFERENCE

Clone Reference

Fei Sun et al. Bioeng Transl Med. 2023;:e10534 Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one-step
Reactivity : Rabbit
Li K et al. Leukemia 2023; 37 (2):308-325 Single-cell analysis reveals the chemotherapy-induced cellular reprogramming and novel therapeutic targets in relapsed/refractory acute myeloid leukemia.
Xiao W et al. Sci Adv 2022; 8 (28):eabn1958 Recombinant DTβ4-inspired porous 3D vascular graft enhanced antithrombogenicity and recruited circulating CD93+/CD34+ cells for endothelialization.
Han C et al. Beijing Da Xue Xue Bao Yi Xue Ban 2022; 54 (6):1151-1157 [Biological characteristics of sheep peripheral blood mesenchymal stem cell].
Khorolskaya JI et al. Biomedicines 2021; 9 (9) Derivation and Characterization of EGFP-Labeled Rabbit Limbal Mesenchymal Stem Cells and Their Potential for Research in Regenerative Ophthalmology.
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REVIEW

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Application Reference
Urbani L et al. PLoS One 2012; 7 (11):e49860 Hypoxia increases mouse satellite cell clone proliferation maintaining both in vitro and in vivo heterogeneity and myogenic potential.
Application : ICC/IF
Peng YJ et al. J Biomed Mater Res A 2012; 100 (5):1126-35 the application of tissue engineering in reversing * c-induced ischemic conjunctiva.
Clone Reference
Fei Sun et al. Bioeng Transl Med. 2023;:e10534 Biomimetic in situ tracheal microvascularization for segmental tracheal reconstruction in one-step
Reactivity : Rabbit
Li K et al. Leukemia 2023; 37 (2):308-325 Single-cell analysis reveals the chemotherapy-induced cellular reprogramming and novel therapeutic targets in relapsed/refractory acute myeloid leukemia.
Xiao W et al. Sci Adv 2022; 8 (28):eabn1958 Recombinant DTβ4-inspired porous 3D vascular graft enhanced antithrombogenicity and recruited circulating CD93+/CD34+ cells for endothelialization.
Han C et al. Beijing Da Xue Xue Bao Yi Xue Ban 2022; 54 (6):1151-1157 [Biological characteristics of sheep peripheral blood mesenchymal stem cell].
Xu H et al. Stem Cells Transl Med 2021; 10 (5):781-796 Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid-induced osteonecrosis of the femoral head.
Kikuchi S et al. Regen Ther 2021; 16:32-41 Development of a nasal mucosa-removal model for evaluating cell therapy.
Chopra H et al. Blood Cells Mol Dis 2021; 86:102487 CD133+CD34+ cells can give rise to EPCs: A comparative rabbit and human study.
Zhen Wang et al. Stem Cells Int 2021; 2021:9947187 Effect of Activated Platelet-Rich Plasma on Chondrogenic Differentiation of Rabbit Bone Marrow-Derived Mesenchymal Stem Cells.
Khorolskaya JI et al. Biomedicines 2021; 9 (9) Derivation and Characterization of EGFP-Labeled Rabbit Limbal Mesenchymal Stem Cells and Their Potential for Research in Regenerative Ophthalmology.
Zhan JW et al. PLoS One 2020; 15 (6):e0234747 Constant compression decreases vascular bud and VEGFA expression in a rabbit vertebral endplate ex vivo culture model.
Nishi H et al. J Am Heart Assoc 2019; 8 (20):e014074 Biodegradable Flow Diverter for the Treatment of Intracranial Aneurysms: A Pilot Study Using a Rabbit Aneurysm Model.
Yeung TL et al. Clin Cancer Res 2019; Anticancer immunotherapy by MFAP5 blockade inhibits fibrosis and enhances chemosensitivity in ovarian and pancreatic cancer.
Niu L et al. J Biomed Mater Res B Appl Biomater 2018; (Epub) PLGA/PLCA casting and PLGA/PDPA electrospinning bilayer film for prevention of postoperative adhesion.
Chen B et al. J Cell Biochem 2018; (Epub) Characterization of the structure of rabbit anterior cruciate ligament and its stem/progenitor cells.
Koon YL et al. Sci Rep 2018; 8 (1):9519 Enhanced Delta-Notch Lateral Inhibition Model Incorporating Intracellular Notch Heterogeneity and Tension-Dependent Rate of Delta-Notch Binding that Reproduces Sprouting Angiogenesis Patterns.
Tiwary S et al. Sci Rep 2018; 8 (1):8267 Metastatic Brain Tumors Disrupt the Blood-Brain Barrier and Alter Lipid Metabolism by Inhibiting Expression of the Endothelial Cell Fatty Acid Transporter Mfsd2a.
Jia Z et al. J Transl Med 2018; 16 (1):123 Repair of articular cartilage defects with intra-articular injection of autologous rabbit synovial fluid-derived mesenchymal stem cells.
Lee HY et al. Oncotarget 2018; 9 (17):13193-13205 EIF3C-enhanced exosome secretion promotes angiogenesis and tumorigenesis of human hepatocellular carcinoma.
Okubo K et al. Nat Med 2018; (Epub) Macrophage extracellular trap formation promoted by platelet activation is a key mediator of rhabdomyolysis-induced acute kidney injury.
Kwiatkowski SC et al. PLoS One 2017; 12 (9):e0185065 Neuropilin-1 modulates TGFβ signaling to drive glioblastoma growth and recurrence after anti-angiogenic therapy.
Ma X et al. J Cell Sci 2017; Nonmuscle myosin II-B regulates epicardial integrity and epicardial derived mesenchymal cell maturation.
Su LJ et al. Sci Rep 2017; 7:45607 Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs.
Jiang P et al. Exp Biol Med (Maywood) 2015; 240 (7):876-83 Phagocytosis of platelets enhances endothelial cell survival under serum deprivation.
Han MS et al. Quant Imaging Med Surg 2014; 4 (3):156-62 Lessons learned from imaging mouse ovarian tumors: the route of probe injection makes a difference.
Hirahashi J et al. Sci Rep 2014; 4:6406 Immunomodulation with eicosapentaenoic acid supports the treatment of autoimmune small-vessel vasculitis.
Yang J et al. J Res Med Sci 2014; 19 (2):112-6 Adipose-derived stem cells from the breast.
Lee TC et al. PLoS One 2014; 9 (11):e111390 Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells.
Choi JH et al. Mol Cells 2014; 37 (6):487-96 Hypoxia-induced endothelial progenitor cell function is blunted in angiotensinogen knockout mice.
Schem C et al. BMC Cancer 2013; 13:32 Preclinical evaluation of * as a single agent in the prophylactic setting in a mouse model of bone metastases.
Uchibori R et al. Cancer Res 2013; 73 (1):364-72 NF-κB activity regulates mesenchymal stem cell accumulation at tumor sites.
Application : IHC
Jung SY et al. J Cell Biochem 2012; 113 (5):1478-87 Decursin inhibits vasculogenesis in early tumor progression by suppression of endothelial progenitor cell differentiation and function.
Kubo N et al. J Gastroenterol Hepatol 2012; 27 (1):165-72 Efficacy of adipose tissue-derived mesenchymal stem cells for fulminant hepatitis in mice induced by concanavalin A.
Tchaicha JH et al. Cancer Res 2011; 71 (20):6371-81 Glioblastoma angiogenesis and tumor cell invasiveness are differentially regulated by β8 integrin.
Tchaicha JH et al. Oncogene 2010; 29 (31):4460-72 A mosaic mouse model of astrocytoma identifies alphavbeta8 integrin as a negative regulator of tumor angiogenesis.
Spirli C et al. Gastroenterology 2010; 138 (1):360-371.e7 ERK1/2-dependent vascular endothelial growth factor signaling sustains cyst growth in polycystin-2 defective mice.
Truman JP et al. PLoS One 2010; 5 (8):e12310 Endothelial membrane remodeling is obligate for anti-angiogenic radiosensitization during tumor radiosurgery.
Application : IHC
Reactivity : Mouse
Hirahashi J et al. Circulation 2009; 120 (13):1255-65 Mac-1 (CD11b/CD18) links inflammation and thrombosis after glomerular injury.
Trempus CS et al. J Invest Dermatol 2003; 120 (4):501-11 Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339