GeneTex
United States (US)

CD34 antibody [MEC14.7]

CD34-antibody-MEC147_FACS_GTX28158-1_18121409_634.jpg

Cat No. GTX28158

Host Rat
Clonality Monoclonal
Clone Name MEC14.7
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, IHC
Reactivity Human, Mouse, Rabbit
APPLICATION

Application Note

For ELISA: Use at an assay dependent dilution. For FACS: Use at a dilution of 1:500. For IF: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1:500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For IHC-Fr: Use at a dilution of 1:500. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:500. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

41 kDa. ( Note )

Specificity/Sensitivity

Reacts with Mouse CD34, a protein present on endothelial cells and hematopoietic progenitor cells. The antibody recognizes a neuraminidase sensitive epitope on endothelium in vivo, particularly on small vessels and neoformed capillaries and developing vascular structures in embryonal structures. Furthermore the antibody reacts with hematopoietic progenitors particularly of myelomonocytic colony forming cells.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline containing 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Protein G affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd34 Molecule,Cd34

Cellular Localization

Type I membrane protein.

Background

The antibody is a useful reagent for identification and characterization of capillary endothelial cells. Furthermore the antibody can be used for isolation and characterization of hematopoietic progenitor cells. In summary the antibody can be used for ELISA, immunofluorescence, immunohistochemistry on frozen and paraffin sections, Western blot, immunoprecipitation, flow cytometry and cell separation. Western blot: The reported molecular mass of CD34 is ranging from 100 to 120 kDa, Apart from the HEV form (Sgp90) which is about 90 kDa. MEC14.7 can recognize a band of about 100 kDa from the H5V line but a band of 75 to 85 kDa from normal tissue. Since different patterns of glycosylation of the molecule are seen with different cell types, this might explain the wide range of CD34 molecular mass (from 75-85 kDa to 120 kD).

Database

Research Area

DATA IMAGES
CD34-antibody-MEC147_FACS_GTX28158-1_18121409_634.jpg

GTX28158 FACS Image

Staining of mouse H5V cells using CD34 antibody (GTX28158) and purified rat IgG2a isotype control.

REFERENCE
Application Reference
Clone Reference
Package List Price ($)
$ 319