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CD34 antibody [MEC14.7]

Anti-CD34 antibody [MEC14.7] used in Flow cytometry (FACS). GTX28158

Cat No. GTX28158

Reference 24
Host Rat
Clonality Monoclonal
Clone Name MEC14.7
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, IHC
Reactivity Human, Mouse, Rabbit
Package
100 μg ($319)
See all CD34 products
APPLICATION

Application Note

For ELISA: Use at an assay dependent dilution. For FACS: Use at a dilution of 1:500. For IF: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1:500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For IHC-Fr: Use at a dilution of 1:500. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:500. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

41 kDa. ( Note )

Specificity/Sensitivity

Reacts with Mouse CD34, a protein present on endothelial cells and hematopoietic progenitor cells. The antibody recognizes a neuraminidase sensitive epitope on endothelium in vivo, particularly on small vessels and neoformed capillaries and developing vascular structures in embryonal structures. Furthermore the antibody reacts with hematopoietic progenitors particularly of myelomonocytic colony forming cells.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline containing 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Protein G affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd34 Molecule,Cd34

Cellular Localization

Type I membrane protein.

Background

The antibody is a useful reagent for identification and characterization of capillary endothelial cells. Furthermore the antibody can be used for isolation and characterization of hematopoietic progenitor cells. In summary the antibody can be used for ELISA, immunofluorescence, immunohistochemistry on frozen and paraffin sections, Western blot, immunoprecipitation, flow cytometry and cell separation. Western blot: The reported molecular mass of CD34 is ranging from 100 to 120 kDa, Apart from the HEV form (Sgp90) which is about 90 kDa. MEC14.7 can recognize a band of about 100 kDa from the H5V line but a band of 75 to 85 kDa from normal tissue. Since different patterns of glycosylation of the molecule are seen with different cell types, this might explain the wide range of CD34 molecular mass (from 75-85 kDa to 120 kD).

Database

Research Area

DATA IMAGES
Anti-CD34 antibody [MEC14.7] used in Flow cytometry (FACS). GTX28158

GTX28158 FACS Image

Staining of mouse H5V cells using CD34 antibody (GTX28158) and purified rat IgG2a isotype control.
REFERENCE

Application Reference

Niu L et al. J Biomed Mater Res B Appl Biomater 2018; (Epub) PLGA/PLCA casting and PLGA/PDPA electrospinning bilayer film for prevention of postoperative adhesion.
Application : IHC-P
Reactivity : Rabbit
Chen B et al. J Cell Biochem 2018; (Epub) Characterization of the structure of rabbit anterior cruciate ligament and its stem/progenitor cells.
Application : IHC-P
Reactivity : Rabbit
Koon YL et al. Sci Rep 2018; 8 (1) : 9519 Enhanced Delta-Notch Lateral Inhibition Model Incorporating Intracellular Notch Heterogeneity and Tension-Dependent Rate of Delta-Notch Binding that Reproduces Sprouting Angiogenesis Patterns.
Application : ICC/IF
Reactivity : Human
Tiwary S et al. Sci Rep 2018; 8 (1) : 8267 Metastatic Brain Tumors Disrupt the Blood-Brain Barrier and Alter Lipid Metabolism by Inhibiting Expression of the Endothelial Cell Fatty Acid Transporter Mfsd2a.
Application : IHC
Reactivity : Mouse
Jia Z et al. J Transl Med 2018; 16 (1) : 123 Repair of articular cartilage defects with intra-articular injection of autologous rabbit synovial fluid-derived mesenchymal stem cells.
Application : FACS
Reactivity : Rabbit
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Application Reference
Niu L et al. J Biomed Mater Res B Appl Biomater 2018; (Epub) PLGA/PLCA casting and PLGA/PDPA electrospinning bilayer film for prevention of postoperative adhesion.
Application : IHC-P
Reactivity : Rabbit
Chen B et al. J Cell Biochem 2018; (Epub) Characterization of the structure of rabbit anterior cruciate ligament and its stem/progenitor cells.
Application : IHC-P
Reactivity : Rabbit
Koon YL et al. Sci Rep 2018; 8 (1):9519 Enhanced Delta-Notch Lateral Inhibition Model Incorporating Intracellular Notch Heterogeneity and Tension-Dependent Rate of Delta-Notch Binding that Reproduces Sprouting Angiogenesis Patterns.
Application : ICC/IF
Reactivity : Human
Tiwary S et al. Sci Rep 2018; 8 (1):8267 Metastatic Brain Tumors Disrupt the Blood-Brain Barrier and Alter Lipid Metabolism by Inhibiting Expression of the Endothelial Cell Fatty Acid Transporter Mfsd2a.
Application : IHC
Reactivity : Mouse
Jia Z et al. J Transl Med 2018; 16 (1):123 Repair of articular cartilage defects with intra-articular injection of autologous rabbit synovial fluid-derived mesenchymal stem cells.
Application : FACS
Reactivity : Rabbit
Lee HY et al. Oncotarget 2018; 9 (17):13193-13205 EIF3C-enhanced exosome secretion promotes angiogenesis and tumorigenesis of human hepatocellular carcinoma.
Application : IHC-P
Reactivity : Mouse
Okubo K et al. Nat Med 2018; (Epub) Macrophage extracellular trap formation promoted by platelet activation is a key mediator of rhabdomyolysis-induced acute kidney injury.
Application : IHC-P
Reactivity : Mouse
Kwiatkowski SC et al. PLoS One 2017; 12 (9):e0185065 Neuropilin-1 modulates TGFβ signaling to drive glioblastoma growth and recurrence after anti-angiogenic therapy.
Application : IHC-P
Ma X et al. J Cell Sci 2017; Nonmuscle myosin II-B regulates epicardial integrity and epicardial derived mesenchymal cell maturation.
Application : IHC-P
Reactivity : Mouse
Jiang P et al. Exp Biol Med (Maywood) 2015; 240 (7):876-83 Phagocytosis of platelets enhances endothelial cell survival under serum deprivation.
Application : IHC
Reactivity : Mouse
Han MS et al. Quant Imaging Med Surg 2014; 4 (3):156-62 Lessons learned from imaging mouse ovarian tumors: the route of probe injection makes a difference.
Application : IHC-P
Reactivity : Human
Hirahashi J et al. Sci Rep 2014; 4:6406 Immunomodulation with eicosapentaenoic acid supports the treatment of autoimmune small-vessel vasculitis.
Application : IHC-P
Reactivity : Mouse
Yang J et al. J Res Med Sci 2014; 19 (2):112-6 Adipose-derived stem cells from the breast.
Application : ICC/IF
Reactivity : Human
Lee TC et al. PLoS One 2014; 9 (11):e111390 Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells.
Application : FACS
Reactivity : Human, Rabbit
Schem C et al. BMC Cancer 2013; 13:32 Preclinical evaluation of * as a single agent in the prophylactic setting in a mouse model of bone metastases.
Urbani L et al. PLoS One 2012; 7 (11):e49860 Hypoxia increases mouse satellite cell clone proliferation maintaining both in vitro and in vivo heterogeneity and myogenic potential.
Application : ICC/IF
Peng YJ et al. J Biomed Mater Res A 2012; 100 (5):1126-35 the application of tissue engineering in reversing * c-induced ischemic conjunctiva.
Tchaicha JH et al. Cancer Res 2011; 71 (20):6371-81 Glioblastoma angiogenesis and tumor cell invasiveness are differentially regulated by β8 integrin.
Application : ICC/IF
Reactivity : Human
Tchaicha JH et al. Oncogene 2010; 29 (31):4460-72 A mosaic mouse model of astrocytoma identifies alphavbeta8 integrin as a negative regulator of tumor angiogenesis.
Spirli C et al. Gastroenterology 2010; 138 (1):360-371.e7 ERK1/2-dependent vascular endothelial growth factor signaling sustains cyst growth in polycystin-2 defective mice.
Application : IHC
Reactivity : Mouse
Hirahashi J et al. Circulation 2009; 120 (13):1255-65 Mac-1 (CD11b/CD18) links inflammation and thrombosis after glomerular injury.
Application : IHC-P
Reactivity : Mouse
Trempus CS et al. J Invest Dermatol 2003; 120 (4):501-11 Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34.
Application : ICC/IF
Reactivity : Mouse
Clone Reference
Uchibori R et al. Cancer Res 2013; 73 (1):364-72 NF-κB activity regulates mesenchymal stem cell accumulation at tumor sites.
Application : IHC
Truman JP et al. PLoS One 2010; 5 (8):e12310 Endothelial membrane remodeling is obligate for anti-angiogenic radiosensitization during tumor radiosurgery.
Application : IHC
Reactivity : Mouse
Package List Price ($)
$ 319