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CD34 antibody [MEC14.7]

Anti-CD34 antibody [MEC14.7] used in Flow cytometry (FACS). GTX28158

Cat No. GTX28158

Reference 30
1

Host

 Rat

Clonality

 Monoclonal

Clone Name

 MEC14.7

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, IHC

Reactivity

 Human, Mouse, Rabbit
Package
100 μg ($379)
See all CD34 products

APPLICATION

Application Note

For ELISA: Use at an assay dependent dilution. For FACS: Use at a dilution of 1:500. For IF: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1:500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For IHC-Fr: Use at a dilution of 1:500. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:500. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

41 kDa. ( Note )

Product Note

Reacts with Mouse CD34, a protein present on endothelial cells and hematopoietic progenitor cells. The antibody recognizes a neuraminidase sensitive epitope on endothelium in vivo, particularly on small vessels and neoformed capillaries and developing vascular structures in embryonal structures. Furthermore the antibody reacts with hematopoietic progenitors particularly of myelomonocytic colony forming cells.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Protein G affinity purified

Conjugation

Unconjugated

RRID

AB_369630

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD34 molecule

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-CD34 antibody [MEC14.7] used in Flow cytometry (FACS). GTX28158

GTX28158 FACS Image

Staining of mouse H5V cells using CD34 antibody (GTX28158) and purified rat IgG2a isotype control.

REFERENCE

Application Reference

Xu H et al. Stem Cells Transl Med 2021; 10 (5) : 781-796 Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid-induced osteonecrosis of the femoral head.
Application : FACS
Reactivity : Rabbit
Kikuchi S et al. Regen Ther 2021; 16 : 32-41 Development of a nasal mucosa-removal model for evaluating cell therapy.
Application : FACS
Reactivity : Rabbit
Chopra H et al. Blood Cells Mol Dis 2021; 86 : 102487 CD133+CD34+ cells can give rise to EPCs: A comparative rabbit and human study.
Application : FACS
Reactivity : Rabbit
Zhan JW et al. PLoS One 2020; 15 (6) : e0234747 Constant compression decreases vascular bud and VEGFA expression in a rabbit vertebral endplate ex vivo culture model.
Application : IHC-P
Reactivity : Rabbit
Nishi H et al. J Am Heart Assoc 2019; 8 (20) : e014074 Biodegradable Flow Diverter for the Treatment of Intracranial Aneurysms: A Pilot Study Using a Rabbit Aneurysm Model.
Application : IHC-Fr
Reactivity : Rabbit
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REVIEW

CD34 antibody [MEC14.7] Cat No. GTX28158

Rating
( Average 5 based on 1 users reviews)
Application
Flow cytometry(FACS)
( Average 5 based on 1 users reviews)
Anti-CD34 antibody [MEC14.7] used in Flow cytometry (FACS). GTX28158
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : FACS
Sample Species : Rb
Figure Note : The data was peer reviewed and published in the journal J Transl Med in 2018. PMID: 29739472
Submit a Review
Application Reference
Xu H et al. Stem Cells Transl Med 2021; 10 (5):781-796 Cotransplantation of mesenchymal stem cells and endothelial progenitor cells for treating steroid-induced osteonecrosis of the femoral head.
Application : FACS
Reactivity : Rabbit
Kikuchi S et al. Regen Ther 2021; 16:32-41 Development of a nasal mucosa-removal model for evaluating cell therapy.
Application : FACS
Reactivity : Rabbit
Chopra H et al. Blood Cells Mol Dis 2021; 86:102487 CD133+CD34+ cells can give rise to EPCs: A comparative rabbit and human study.
Application : FACS
Reactivity : Rabbit
Zhan JW et al. PLoS One 2020; 15 (6):e0234747 Constant compression decreases vascular bud and VEGFA expression in a rabbit vertebral endplate ex vivo culture model.
Application : IHC-P
Reactivity : Rabbit
Nishi H et al. J Am Heart Assoc 2019; 8 (20):e014074 Biodegradable Flow Diverter for the Treatment of Intracranial Aneurysms: A Pilot Study Using a Rabbit Aneurysm Model.
Application : IHC-Fr
Reactivity : Rabbit
Yeung TL et al. Clin Cancer Res 2019; Anticancer immunotherapy by MFAP5 blockade inhibits fibrosis and enhances chemosensitivity in ovarian and pancreatic cancer.
Niu L et al. J Biomed Mater Res B Appl Biomater 2018; (Epub) PLGA/PLCA casting and PLGA/PDPA electrospinning bilayer film for prevention of postoperative adhesion.
Application : IHC-P
Reactivity : Rabbit
Chen B et al. J Cell Biochem 2018; (Epub) Characterization of the structure of rabbit anterior cruciate ligament and its stem/progenitor cells.
Application : IHC-P
Reactivity : Rabbit
Koon YL et al. Sci Rep 2018; 8 (1):9519 Enhanced Delta-Notch Lateral Inhibition Model Incorporating Intracellular Notch Heterogeneity and Tension-Dependent Rate of Delta-Notch Binding that Reproduces Sprouting Angiogenesis Patterns.
Application : ICC/IF
Reactivity : Human
Tiwary S et al. Sci Rep 2018; 8 (1):8267 Metastatic Brain Tumors Disrupt the Blood-Brain Barrier and Alter Lipid Metabolism by Inhibiting Expression of the Endothelial Cell Fatty Acid Transporter Mfsd2a.
Application : IHC
Reactivity : Mouse
Jia Z et al. J Transl Med 2018; 16 (1):123 Repair of articular cartilage defects with intra-articular injection of autologous rabbit synovial fluid-derived mesenchymal stem cells.
Application : FACS
Reactivity : Rabbit
Lee HY et al. Oncotarget 2018; 9 (17):13193-13205 EIF3C-enhanced exosome secretion promotes angiogenesis and tumorigenesis of human hepatocellular carcinoma.
Application : IHC-P
Reactivity : Mouse
Okubo K et al. Nat Med 2018; (Epub) Macrophage extracellular trap formation promoted by platelet activation is a key mediator of rhabdomyolysis-induced acute kidney injury.
Application : IHC-P
Reactivity : Mouse
Kwiatkowski SC et al. PLoS One 2017; 12 (9):e0185065 Neuropilin-1 modulates TGFβ signaling to drive glioblastoma growth and recurrence after anti-angiogenic therapy.
Application : IHC-P
Ma X et al. J Cell Sci 2017; Nonmuscle myosin II-B regulates epicardial integrity and epicardial derived mesenchymal cell maturation.
Application : IHC-P
Reactivity : Mouse
Jiang P et al. Exp Biol Med (Maywood) 2015; 240 (7):876-83 Phagocytosis of platelets enhances endothelial cell survival under serum deprivation.
Application : IHC
Reactivity : Mouse
Han MS et al. Quant Imaging Med Surg 2014; 4 (3):156-62 Lessons learned from imaging mouse ovarian tumors: the route of probe injection makes a difference.
Application : IHC-P
Reactivity : Human
Hirahashi J et al. Sci Rep 2014; 4:6406 Immunomodulation with eicosapentaenoic acid supports the treatment of autoimmune small-vessel vasculitis.
Application : IHC-P
Reactivity : Mouse
Yang J et al. J Res Med Sci 2014; 19 (2):112-6 Adipose-derived stem cells from the breast.
Application : ICC/IF
Reactivity : Human
Lee TC et al. PLoS One 2014; 9 (11):e111390 Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells.
Application : FACS
Reactivity : Human, Rabbit
Schem C et al. BMC Cancer 2013; 13:32 Preclinical evaluation of * as a single agent in the prophylactic setting in a mouse model of bone metastases.
Urbani L et al. PLoS One 2012; 7 (11):e49860 Hypoxia increases mouse satellite cell clone proliferation maintaining both in vitro and in vivo heterogeneity and myogenic potential.
Application : ICC/IF
Peng YJ et al. J Biomed Mater Res A 2012; 100 (5):1126-35 the application of tissue engineering in reversing * c-induced ischemic conjunctiva.
Tchaicha JH et al. Cancer Res 2011; 71 (20):6371-81 Glioblastoma angiogenesis and tumor cell invasiveness are differentially regulated by β8 integrin.
Application : ICC/IF
Reactivity : Human
Tchaicha JH et al. Oncogene 2010; 29 (31):4460-72 A mosaic mouse model of astrocytoma identifies alphavbeta8 integrin as a negative regulator of tumor angiogenesis.
Spirli C et al. Gastroenterology 2010; 138 (1):360-371.e7 ERK1/2-dependent vascular endothelial growth factor signaling sustains cyst growth in polycystin-2 defective mice.
Application : IHC
Reactivity : Mouse
Hirahashi J et al. Circulation 2009; 120 (13):1255-65 Mac-1 (CD11b/CD18) links inflammation and thrombosis after glomerular injury.
Application : IHC-P
Reactivity : Mouse
Trempus CS et al. J Invest Dermatol 2003; 120 (4):501-11 Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34.
Application : ICC/IF
Reactivity : Mouse
Clone Reference
Uchibori R et al. Cancer Res 2013; 73 (1):364-72 NF-κB activity regulates mesenchymal stem cell accumulation at tumor sites.
Application : IHC
Truman JP et al. PLoS One 2010; 5 (8):e12310 Endothelial membrane remodeling is obligate for anti-angiogenic radiosensitization during tumor radiosurgery.
Application : IHC
Reactivity : Mouse
SDS
Sodium Azide.pdf
Package List Price ($)
$ 379