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CD34 antibody [MEC14.7]

Anti-CD34 antibody [MEC14.7] used in Flow cytometry (FACS). GTX28158

Cat No. GTX28158

Host

Rat

Clonality

Monoclonal

Clone Name

MEC14.7

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, IHC

Reactivity

Human, Mouse, Rabbit
Package
100 μg ($379)

APPLICATION

Application Note

For ELISA: Use at an assay dependent dilution. For FACS: Use at a dilution of 1:500. For IF: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1:500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For IHC-Fr: Use at a dilution of 1:500. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:500. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

41 kDa. ( Note )

Product Note

Reacts with Mouse CD34, a protein present on endothelial cells and hematopoietic progenitor cells. The antibody recognizes a neuraminidase sensitive epitope on endothelium in vivo, particularly on small vessels and neoformed capillaries and developing vascular structures in embryonal structures. Furthermore the antibody reacts with hematopoietic progenitors particularly of myelomonocytic colony forming cells.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Protein G affinity purified

Conjugation

Unconjugated

RRID

AB_369630

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD34 molecule

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-CD34 antibody [MEC14.7] used in Flow cytometry (FACS). GTX28158

GTX28158 FACS Image

Staining of mouse H5V cells using CD34 antibody (GTX28158) and purified rat IgG2a isotype control.

REFERENCE

REVIEW

CD34 antibody [MEC14.7] Cat No. GTX28158

Rating ( Average 5 based on 1 users reviews)
Application
Flow cytometry(FACS) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : FACS
Sample Species : Rb
Figure Note : The data was peer reviewed and published in the journal J Transl Med in 2018. PMID: 29739472
Application Reference
Clone Reference
SDS
Sodium Azide.pdf
Package List Price ($)
$ 379