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CD34 antibody [QBEnd/10]

Anti-CD34 antibody [QBEnd/10] used in IHC (Paraffin sections) (IHC-P). GTX42056

Cat. No. GTX42056

Host

Mouse

Clonality

Monoclonal

Clone Name

QBEnd/10

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, EM

Reactivity

Human, Cynomolgus monkey, Rhesus Monkey
Package
200 μg ($459)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1/500-1/1,000
IHC-Fr Assay dependent
FACS 1/100 ­ 1/200
IP Assay dependent
EM Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Product Note

This antibody recognizes the human CD34. Clone QBEND/10 has been classified as binding to the class II epitope, resistant to neuraminidase treatment but sensitive to both glycoprotease and chymopapain digestion.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human endothelial cell membrane vesicles.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD34 molecule

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-CD34 antibody [QBEnd/10] used in IHC (Paraffin sections) (IHC-P). GTX42056

GTX42056 IHC-P Image

IHC-P analysis of placenta tissue using GTX42056 CD34 Class II antibody [QBEND/10].

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459