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CD35 antibody [CR1/802]

Anti-CD35 antibody [CR1/802] used in IHC (Paraffin sections) (IHC-P). GTX34504
Anti-CD35 antibody [CR1/802] used in IHC (Paraffin sections) (IHC-P). GTX34504

Cat. No. GTX34504

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 CR1/802

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Monkey, Baboon
Package
100 μg ($529)
See all CD35 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

224 kDa. ( Note )

Product Note

Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This MAb does not block CR1 activity. It is highly specific to CR1 and shows no cross-reaction with CR2.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human CR1 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C3BR , C4BR , CD35 , CR1 , KN , complement C3b/C4b receptor 1 (Knops blood group)

Cellular Localization

Membrane

Background

This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD35 antibody [CR1/802] used in IHC (Paraffin sections) (IHC-P). GTX34504

GTX34504 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34504 CD35 antibody [CR1/802].

Anti-CD35 antibody [CR1/802] used in IHC (Paraffin sections) (IHC-P). GTX34504

GTX34504 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34504 CD35 antibody [CR1/802].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529