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CD35 antibody [E11]

Anti-CD35 antibody [E11] used in Flow cytometry (FACS). GTX76302
Anti-CD35 antibody [E11] used in Flow cytometry (FACS). GTX76302

Cat No. GTX76302

Host

Mouse

Clonality

Monoclonal

Clone Name

E11

Isotype

IgG1

Application

WB, IHC-P, IHC-Fr, FACS

Reactivity

Human, Baboon, Cynomolgus monkey, Rhesus Monkey
Package
100 μg ($299)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P 1-10μg/ml
IHC-Fr 1-10μg/ml
FACS 1/50-1/200

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.

Not tested in other applications.

Calculated MW

224 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human acute monocytic leukaemia cells.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Complement C3B/C4B Receptor 1 (Knops Blood Group) , C3Br , C4Br , Cd35 , Kn , Cr1

Background

This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD35 antibody [E11] used in Flow cytometry (FACS). GTX76302

GTX76302 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX76302 CD35 antibody [E11].

Anti-CD35 antibody [E11] used in Flow cytometry (FACS). GTX76302

GTX76302 FACS Image

FACS analysis of human peripheral blood monocytes using GTX76302 CD35 antibody [E11].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf