GeneTex
United States (US)

CD35 antibody [SPM554]

GTX34503_20170712_IHC-P_18121410_495.jpg

Cat No. GTX34503

Host Mouse
Clonality Monoclonal
Clone Name SPM554
Isotype IgG1
Application IHC-P
Reactivity Human, Baboon, Cynomolgus monkey, Rhesus Monkey
APPLICATION

Application Note

IHC-P:0.5-1ug/ml for 30 minutes at RT.

Calculated MW

224 kDa. ( Note )

Specificity/Sensitivity

Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This MAb is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This MAb is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein.ĀFollicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This MAb labels follicular dendritic cells and follicular dendritic cell sarcoma.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Intact human monocytes

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C4BR Antibody , complement component 3b/4b receptor 1 (Knops blood group) Antibody , C3BR Antibody , CR1 Antibody , KN Antibody

Background

This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX34503_20170712_IHC-P_18121410_495.jpg

GTX34503 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with CD35 Monoclonal Antibody (SPM554).

Package List Price ($)
$ 329