GeneTex
United States (US)

CD36 antibody [C1C3]

GTX100642_39974_IFA_mESC_19011506_903.jpg
GTX100642_IHC_19011506_397.jpg
GTX100642_42298_20151203_WB_M_adipose_19011506_554.jpg
GTX100642_39974_WB_19011506_805.jpg

Cat No. GTX100642

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

K562 , mouse brown adipose , *RAW264.7

Predict Reactivity

Rat, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CD36. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd36 Molecule,Bdplt10,Chds7,Fat,Gp3B,Gp4,Gpiv,Pasiv,Scarb3,Cd36

Cellular Localization

Membrane

Background

The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100642_39974_IFA_mESC_19011506_903.jpg

GTX100642 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using CD36(GTX100642) antibody at 1:200 dilution.

GTX100642_IHC_19011506_397.jpg

GTX100642 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using CD36(GTX100642) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100642_42298_20151203_WB_M_adipose_19011506_554.jpg

GTX100642 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD36 antibody [C1C3] (GTX100642) diluted at 1:1000.

GTX100642_39974_WB_19011506_805.jpg

GTX100642 WB Image

Sample (30 µg of whole cell lysate)
A: K562
10% SDS PAGE
GTX100642 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

CD36 antibody [C1C3] Cat No. GTX100642

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 15-Oct-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW264.7 cells
Amount used : 30μg
Blocking : 5% Skim milk in TBST(0.1% tween-20), 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169