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CD36 antibody [C1C3]

Orthogonal
Anti-CD36 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100642
Anti-CD36 antibody [C1C3] used in Western Blot (WB). GTX100642
Anti-CD36 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100642
Anti-CD36 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100642
Anti-CD36 antibody [C1C3] used in Western Blot (WB). GTX100642
Anti-CD36 antibody [C1C3] used in Western Blot (WB). GTX100642

Cat No. GTX100642

Reference 13
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all CD36 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

K562 , mouse brown adipose , *RAW264.7

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CD36. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949927

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD36 molecule , BDPLT10 , CHDS7 , FAT , GP3B , GP4 , GPIV , PASIV , SCARB3

Cellular Localization

Membrane

Background

The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD36 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100642

GTX100642 IHC-P Image

CD36 antibody [C1C3] detects CD36 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
CD36 stained by CD36 antibody [C1C3] (GTX100642) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD36 antibody [C1C3] used in Western Blot (WB). GTX100642

GTX100642 WB Image

Orthogonal

Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD36 antibody [C1C3] (GTX100642) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)

Anti-CD36 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100642

GTX100642 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using CD36(GTX100642) antibody at 1:200 dilution.

Anti-CD36 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100642

GTX100642 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using CD36(GTX100642) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD36 antibody [C1C3] used in Western Blot (WB). GTX100642

GTX100642 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD36 antibody [C1C3] (GTX100642) diluted at 1:1000.

Anti-CD36 antibody [C1C3] used in Western Blot (WB). GTX100642

GTX100642 WB Image

Sample (30 μg of whole cell lysate)
A: K562
10% SDS PAGE
GTX100642 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Njau F et al. Antioxidants (Basel) 2021; 10 (11) Calcium Dobesilate Modulates PKCδ-NADPH Oxidase- MAPK-NF-κB Signaling Pathway to Reduce CD14, TLR4, and MMP9 Expression during Monocyte-to-Macrophage Differentiation: Potential Therapeutic Implications for Atherosclerosis.
Application : WB
Reactivity : Human
Tsai YT et al. Nutrients 2020; 12 (3) Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice.
Brusini R et al. Int J Pharm 2020; 581 : 119282 Squalene-based nanoparticles for the targeting of atherosclerotic lesions.
Application : IHC-P
Reactivity : Mouse
Tsukahara T et al. Cell Signal 2020; 65 : 109459 1-O-alkyl glycerophosphate-induced CD36 expression drives oxidative stress in microglial cells.
Application : WB
Reactivity : Mouse
Xu M et al. Cardiovasc Res 2018; (Epub) Choline ameliorates cardiac hypertrophy by regulating metabolic remodelling and UPRmt through SIRT3-AMPK pathway.
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Submit a Reference

REVIEW

CD36 antibody [C1C3] Cat No. GTX100642

Rating
( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 2 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-CD36 antibody [C1C3] used in Western Blot (WB). GTX100642
Date : Anonymous submitted on 20-May-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : Brown adipocytes extract
Amount used : 50 μg
Blocking : 5% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
Figure Note : CL: CL316243, adrenergic-beta 3 receptor agonist
Anti-CD36 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100642
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nutrients in 2020. PMID: 32164196
Anti-CD36 antibody [C1C3] used in Western Blot (WB). GTX100642
Date : Anonymous submitted on 15-Oct-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW264.7 cells
Amount used : 30μg
Blocking : 5% Skim milk in TBST(0.1% tween-20), 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Njau F et al. Antioxidants (Basel) 2021; 10 (11) Calcium Dobesilate Modulates PKCδ-NADPH Oxidase- MAPK-NF-κB Signaling Pathway to Reduce CD14, TLR4, and MMP9 Expression during Monocyte-to-Macrophage Differentiation: Potential Therapeutic Implications for Atherosclerosis.
Application : WB
Reactivity : Human
Tsai YT et al. Nutrients 2020; 12 (3) Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice.
Brusini R et al. Int J Pharm 2020; 581:119282 Squalene-based nanoparticles for the targeting of atherosclerotic lesions.
Application : IHC-P
Reactivity : Mouse
Tsukahara T et al. Cell Signal 2020; 65:109459 1-O-alkyl glycerophosphate-induced CD36 expression drives oxidative stress in microglial cells.
Application : WB
Reactivity : Mouse
Xu M et al. Cardiovasc Res 2018; (Epub) Choline ameliorates cardiac hypertrophy by regulating metabolic remodelling and UPRmt through SIRT3-AMPK pathway.
Sun Q et al. Gene 2018; (Epub) Hypermethylated CD36 gene affected the progression of lung cancer.
Application : WB
Reactivity : Human
Gui Y et al. Cardiovasc Res 2016; A novel small molecule liver X receptor transcriptional regulator, nagilactone B, suppresses atherosclerosis in apoE-deficient mice.
Application : WB
Reactivity : Mouse
Gui YZ et al. Acta Pharmacol Sin 2016; (Epub) Betulin attenuates atherosclerosis in apoE-/- mice by up-regulating ABCA1 and ABCG1.
Application : WB
Reactivity : Human, Mouse
Talukder MA et al. Physiol Rep 2016; 4 (6) heterozygous caveolin-3 mice show increased susceptibility to palmitate-induced * resistance.
Application : WB
Reactivity : Mouse
Du RH et al. Atherosclerosis 2014; 234 (1):120-8 Fumigaclavine C activates PPARγ pathway and attenuates atherogenesis in ApoE-deficient mice.
Application : WB
Reactivity : Mouse
Vaillant F et al. Am J Physiol Heart Circ Physiol 2014; 306 (1):H78-87 Mouse strain differences in metabolic fluxes and function of ex vivo working hearts.
Application : WB
Reactivity : Mouse
Kiyan Y et al. J Mol Cell Cardiol 2014; 66:72-82 oxLDL induces inflammatory responses in vascular smooth muscle cells via * receptor association with CD36 and TLR4.
Application : WB, ICC/IF
Reactivity : Human
Kouadir M et al. PLoS One 2012; 7 (1):e30756 CD36 participates in PrP(106-126)-induced activation of microglia.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169