APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
The monoclonal antibody VM58 is directed against the CD36 antigen, a 90kD glycoprotein, also called GP IIIb expressed on human platelets. The GP IV (GP IIIb) molecule has been shown to function as thrombospondin receptor on platelets and monocytes and as receptor for Plasmodium falciparum infected erythrocytes. The monoclonal antibody reacts with platelets, monocytes, macrophages, erythroblasts and (weakly) with B-cells. In immunohistology the monoclonal antibody reacts with some endothelial cells, adipocytes and the granular layer of the skin. The antibody VM58 induces Fc-receptor-dependent platelet aggregation after binding to platelet. The heterogeneity of the response found in healthy individuals is dependent on the polymorphism of the Fc RII on platelets.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
3mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human monocytes.
Purification
IgG fraction
Ammonium sulfate + DEAE-cellulose, gradient elution
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD36 molecule , BDPLT10 , CHDS7 , FAT , GP3B , GP4 , GPIV , PASIV , SCARB3
Cellular Localization
Cell membrane , Golgi apparatus , Apical cell membrane
Background
The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2014]
Database
Research Area
DATA IMAGES
![Anti-CD36 antibody [VM58] used in Immunohistochemistry (IHC). GTX28140](/templates/default/js/lightbox/images/loading.gif "Anti-CD36 antibody [VM58] used in Immunohistochemistry (IHC). GTX28140")
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GTX28140 IHC Image
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REFERENCE
There are currently no references for CD36 antibody [VM58] (GTX28140). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD36 antibody [VM58] (GTX28140). Be the first to share your experience with this product.
![Anti-CD36 antibody [VM58] used in Immunohistochemistry (IHC). GTX28140](/upload/website/prouct_img/small/GTX28140/img18160_w_23060722_991.webp "Anti-CD36 antibody [VM58] used in Immunohistochemistry (IHC). GTX28140")
![Anti-CD36 antibody [VM58] used in Immunohistochemistry (IHC). GTX28140](/upload/website/prouct_img/normal/GTX28140/img18160_w_23060722_991.webp "Anti-CD36 antibody [VM58] used in Immunohistochemistry (IHC). GTX28140")