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CD36 antibody

Orthogonal
Anti-CD36 antibody used in Western Blot (WB). GTX112891
Anti-CD36 antibody used in IHC (Paraffin sections) (IHC-P). GTX112891
Anti-CD36 antibody used in IHC (Paraffin sections) (IHC-P). GTX112891
Anti-CD36 antibody used in Western Blot (WB). GTX112891
Anti-CD36 antibody used in Western Blot (WB). GTX112891
Anti-CD36 antibody used in Western Blot (WB). GTX112891

Cat. No. GTX112891

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CD36 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

Mouse brown adipose

Predict Reactivity

Rat, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CD36. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731492

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD36 molecule , BDPLT10 , CHDS7 , FAT , GP3B , GP4 , GPIV , PASIV , SCARB3

Cellular Localization

Membrane

Background

The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD36 antibody used in Western Blot (WB). GTX112891

GTX112891 WB Image

Orthogonal

Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD36 antibody (GTX112891) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)

Anti-CD36 antibody used in IHC (Paraffin sections) (IHC-P). GTX112891

GTX112891 IHC-P Image

CD36 antibody detects CD36 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
CD36 stained by CD36 antibody (GTX112891) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD36 antibody used in IHC (Paraffin sections) (IHC-P). GTX112891

GTX112891 IHC-P Image

CD36 antibody detects CD36 protein at cytosol and weak membrane on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
CD36 antibody (GTX112891) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD36 antibody used in Western Blot (WB). GTX112891

GTX112891 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brown adipose
7.5% SDS PAGE
GTX112891 diluted at 1:1000

Anti-CD36 antibody used in Western Blot (WB). GTX112891

GTX112891 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD36 antibody (GTX112891) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD36 antibody used in Western Blot (WB). GTX112891

GTX112891 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD36 antibody (GTX112891) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169