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CD4 antibody [CA-4]

Anti-CD4 antibody [CA-4] used in Flow cytometry (FACS). GTX10882

Cat. No. GTX10882

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 CA-4

Isotype

 IgG1

Application

 ICC/IF, IHC-Fr, FACS

Reactivity

 Human
Package
100 μg ($449)
See all CD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 0.5-1μg/ml
IHC-Fr 1μg/ml
FACS 1-3μg/1x10⁶cells
Not tested in other applications.

Calculated MW

51.11 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites, 1.2% Sodium acetate, 2mg BSA

Preservative

0.01mg Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cell membrane

Background

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-CD4 antibody [CA-4] used in Flow cytometry (FACS). GTX10882

GTX10882 FACS Image

FACS analysis of human PBMC cells using GTX10882 CD4 antibody [CA-4].
Blue : Primary antibody
Green : Isotype control
Red : Cell only control
Antibody amount : 1μg/1x10⁶ cells for 30 min at 20ºC

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 449