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CD4 antibody [GK1.5] (APC)

Anti-CD4 antibody [GK1.5] (APC) used in Flow cytometry (FACS). GTX80146

Cat. No. GTX80146

Reference 7

Host

 Rat

Clonality

 Monoclonal

Clone Name

 GK1.5

Isotype

 IgG2b

Application

 FACS

Reactivity

 Mouse, Golden Syrian Hamster
Package
100 μg ($349)
See all CD4 products
PRODUCT

Summary

The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Product Note

This antibody reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse CTL clone V4 cells

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD4 antigen , L3T4 , Ly-4

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-CD4 antibody [GK1.5] (APC) used in Flow cytometry (FACS). GTX80146

GTX80146 FACS Image

FACS analysis of C57Bl/6 splenocytes using GTX80146 CD4 antibody [GK1.5] (APC).
Solid line : Primary antibody
Dashed line : APC rat IgG2b isotype control
Antibody amount : 0.06 μg

REFERENCE

Clone Reference

Chi-Shuan Fan et al. Cells 2022; 11 (2) Extracellular HSP90α Induces MyD88-IRAK Complex-Associated IKKα/β-NF-κB/IRF3 and JAK2/TYK2-STAT-3 Signaling in Macrophages for Tumor-Promoting M2-Polarization.
Qizhi Ma et al. Precis Clin Med 2021; 4 (3):155-167 CXCL13 expression in mouse 4T1 breast cancer microenvironment elicits antitumor immune response by regulating immune cell infiltration.
Fu Y et al. Mol Carcinog 2019; Downregulation of CPA4 inhibits non small-cell lung cancer growth by suppressing the AKT/c-MYC pathway.
Huang CP et al. Cytotherapy 2017; (Epub) The anti-tumor effect of intravesical administration of normal urothelial cells on bladder cancer.
Das S et al. Proc Natl Acad Sci U S A 2016; 113 (46):13132-13137 GSDMB induces an asthma phenotype characterized by increased airway responsiveness and remodeling without lung inflammation.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 349