GeneTex

  • Country / Location Selection

United States (US)

CD4 antibody [GK1.5]

Cat. No. GTX44531

Reference 21

Host

 Rat

Clonality

 Monoclonal

Clone Name

 GK1.5

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, Depletion, Neutralizing/Inhibition

Reactivity

 Mouse, Golden Syrian Hamster
Package
100 μg ($339)
See all CD4 products
PRODUCT

Summary

The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Depletion Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Murine CD4.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_11172175

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD4 antigen , L3T4 , Ly-4

Cellular Localization

Cell membrane

Database

Research Area

REFERENCE

Application Reference

Chi-Shuan Fan et al. Cells 2022; 11 (2) Extracellular HSP90α Induces MyD88-IRAK Complex-Associated IKKα/β-NF-κB/IRF3 and JAK2/TYK2-STAT-3 Signaling in Macrophages for Tumor-Promoting M2-Polarization.
Application : ICC/IF
Reactivity : Mouse
Qizhi Ma et al. Precis Clin Med 2021; 4 (3) : 155-167 CXCL13 expression in mouse 4T1 breast cancer microenvironment elicits antitumor immune response by regulating immune cell infiltration.
Application : IHC-Fr
Reactivity : Mouse
Fu Y et al. Mol Carcinog 2019; Downregulation of CPA4 inhibits non small-cell lung cancer growth by suppressing the AKT/c-MYC pathway.
Application : IHC-P
Reactivity : Mouse

Clone Reference

2020; In situ vaccination at a peripheral tumor site augments response against melanoma brain metastases
Application : Depletion
Reactivity : Mouse
Lu Xu et al. J Exp Clin Cancer Res. 2018; 37 (1):261 Apigenin suppresses PD-L1 expression in melanoma and host dendritic cells to elicit synergistic therapeutic effects.
Application : FACS
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

There are currently no reviews for CD4 antibody [GK1.5] (GTX44531). Be the first to share your experience with this product.
Submit a Review
Application Reference
Chi-Shuan Fan et al. Cells 2022; 11 (2) Extracellular HSP90α Induces MyD88-IRAK Complex-Associated IKKα/β-NF-κB/IRF3 and JAK2/TYK2-STAT-3 Signaling in Macrophages for Tumor-Promoting M2-Polarization.
Application : ICC/IF
Reactivity : Mouse
Qizhi Ma et al. Precis Clin Med 2021; 4 (3):155-167 CXCL13 expression in mouse 4T1 breast cancer microenvironment elicits antitumor immune response by regulating immune cell infiltration.
Application : IHC-Fr
Reactivity : Mouse
Fu Y et al. Mol Carcinog 2019; Downregulation of CPA4 inhibits non small-cell lung cancer growth by suppressing the AKT/c-MYC pathway.
Application : IHC-P
Reactivity : Mouse
Huang CP et al. Cytotherapy 2017; (Epub) The anti-tumor effect of intravesical administration of normal urothelial cells on bladder cancer.
Application : IHC-P
Reactivity : Mouse
Das S et al. Proc Natl Acad Sci U S A 2016; 113 (46):13132-13137 GSDMB induces an asthma phenotype characterized by increased airway responsiveness and remodeling without lung inflammation.
Application : IHC-P
Reactivity : Mouse
Liu YJ et al. PLoS One 2015; 10 (9):e0136214 Dendritic Cells Regulate Treg-Th17 Axis in Obstructive Phase of Bile Duct Injury in Murine Biliary Atresia.
Application : IHC-Fr
Reactivity : Mouse
Miller M et al. J Immunol 2014; 192 (8):3475-87 ORMDL3 transgenic mice have increased airway remodeling and airway responsiveness characteristic of asthma.
Application : IHC-P
Reactivity : Mouse
Clone Reference
2020; In situ vaccination at a peripheral tumor site augments response against melanoma brain metastases
Application : Depletion
Reactivity : Mouse
Valerio Leoni et al. PLoS Pathog 2018; 14 (8):e1007209 A fully-virulent retargeted oncolytic HSV armed with IL-12 elicits local immunity and vaccine therapy towards distant tumors.
Application : FACS
Reactivity : Mouse
Lu Xu et al. J Exp Clin Cancer Res. 2018; 37 (1):261 Apigenin suppresses PD-L1 expression in melanoma and host dendritic cells to elicit synergistic therapeutic effects.
Application : FACS
Reactivity : Mouse
2017; Intestinal microbiota link lymphopenia to murine autoimmunity via PD-1 + CXCR5 -/dim B-helper T cell induction
Application : IHC-Fr
Reactivity : Mouse
2016; Multispectral Imaging of T and B Cells in Murine Spleen and Tumor
Application : IHC-P
2014; Polarized axonal surface expression of neuronal KCNQ potassium channels is regulated by calmodulin interaction with KCNQ2 subunit
Application : WB, IP
2012; Subcellular distribution of Lck during CD4 T-cell maturation in the thymic medulla regulates the T-cell activation threshold
Application : IP
Reactivity : Mouse
2012; Anti-IL-7 receptor-α reverses established type 1 diabetes in nonobese diabetic mice by modulating effector T-cell function
Application : FACS
Reactivity : Mouse
2012; Nab2 regulates secondary CD8+ T-cell responses through control of TRAIL expression
Application : Depletion
Reactivity : Mouse
2012; Detection of an autoreactive T-cell population within the polyclonal repertoire that undergoes distinct autoimmune regulator (Aire)-mediated selection
Application : FACS, Depletion
Reactivity : Mouse
2011; Type I interferon is selectively required by dendritic cells for immune rejection of tumors
Application : Neutralizing/Inhibition
Reactivity : Mouse
2011; T cells are not required for pathogenesis in the Syrian hamster model of hantavirus pulmonary syndrome
Application : FACS
Reactivity : Golden Syrian Hamster
2008; Regulated movement of CD4 in and out of the immunological synapse
Application : ICC/IF, IP
Reactivity : Mouse
2007; Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes
Application : Neutralizing/Inhibition
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339