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CD4 antibody [GK1.5]

Cat No. GTX44531

Host

Rat

Clonality

Monoclonal

Clone Name

GK1.5

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, Depletion, Neutralizing/Inhibition

Reactivity

Human, Mouse, Golden Syrian Hamster
Package
100 μg ($339)
PRODUCT

Summary

The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Depletion Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10 mM NaH₂PO₄ (pH 7.2), 150 mM NaCl, 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml

Antigen Species

Mouse

Immunogen

Murine CD4.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_11172175

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD4 antigen , L3T4 , Ly-4

Cellular Localization

Cell membrane

Database

Research Area

REFERENCE

REVIEW

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Application Reference
Clone Reference
SDS
PBS.pdf
Sodium Azide.pdf