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CD4 antibody [GK1.5]

Cat No. GTX44531

Host

Rat

Clonality

Monoclonal

Clone Name

GK1.5

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, Depletion, Neutralizing/Inhibition

Reactivity

Human, Mouse, Golden Syrian Hamster
Package
100 μg ($269)
PRODUCT

Summary

The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Depletion Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10 mM NaH₂PO₄ (pH 7.2), 150 mM NaCl, 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml

Antigen Species

Mouse

Immunogen

Murine CD4.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

REFERENCE

REVIEW

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Application Reference
Clone Reference
SDS
PBS.pdf
Sodium Azide.pdf