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CD4 antibody [RM4-5] (FITC)

Anti-CD4 antibody [RM4-5] (FITC) used in Flow cytometry (FACS). GTX43601

Cat. No. GTX43601

Host

 Rat

Clonality

 Monoclonal

Clone Name

 RM4-5

Isotype

 IgG2a

Application

 FACS

Reactivity

 Mouse
Package
100 μg ($399)
See all CD4 products
PRODUCT

Summary

The RM4-5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta2-domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

BALB/c mouse thymocytes

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC) Wavelength

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD4 antigen , L3T4 , Ly-4

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-CD4 antibody [RM4-5] (FITC) used in Flow cytometry (FACS). GTX43601

GTX43601 FACS Image

FACS analysis of C57Bl/6 splenocytes using GTX43601 CD4 antibody [RM4-5] (FITC).
Solid line : Primary antibody
Dashed line : FITC rat IgG2a isotype control
Antibody amount : 0.25 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399