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CD4 antibody [RPA-T4] (Low endotoxin, azide free)

Anti-CD4 antibody [RPA-T4] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX44537
Anti-CD4 antibody [RPA-T4] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX44537
Anti-CD4 antibody [RPA-T4] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX44537
Anti-CD4 antibody [RPA-T4] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX44537

Cat. No. GTX44537

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 RPA-T4

Isotype

 IgG1

Application

 IHC-Fr, FACS, Neutralizing/Inhibition

Reactivity

 Human
Package
100 μg ($399)
See all CD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Fr 1/200-1/2,000
FACS 1/50-1/100
Neutralizing/Inhibition Assay dependent

Note :

IHC-Fr
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections.

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.

Neutralizing/Inhibition
Mouse anti human CD4 antibody, clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation (Piatier-Tonneau et al, 1997).
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Product Note

Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1 of the extracellular region of the CD4 molecule.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human PHA blasts.

Purification

Protein A purified
From tissue culture supernatant

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD4 molecule , CD4mut

Cellular Localization

Cell membrane

Background

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-CD4 antibody [RPA-T4] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX44537

GTX44537 IHC-Fr Image

IHC-Fr analysis of human tonsil tissue using GTX44537 CD4 antibody [RPA-T4] (Low endotoxin, azide free).

Anti-CD4 antibody [RPA-T4] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX44537

GTX44537 IHC-Fr Image

IHC-Fr analysis of human tonsil tissue using GTX44537 CD4 antibody [RPA-T4] (Low endotoxin, azide free).

Anti-CD4 antibody [RPA-T4] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX44537

GTX44537 IHC-Fr Image

IHC-Fr analysis of human tonsil tissue using GTX44537 CD4 antibody [RPA-T4] (Low endotoxin, azide free).

Anti-CD4 antibody [RPA-T4] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX44537

GTX44537 IHC-Fr Image

IHC-Fr analysis of human tonsil tissue using GTX44537 CD4 antibody [RPA-T4] (Low endotoxin, azide free).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations