CD4 antibody [RPA-T4] (PE)

Cat. No. GTX74819

Host	Mouse
Clonality	Monoclonal
Clone Name	RPA-T4
Isotype	IgG1
Application	FACS
Reactivity	Human, Chimpanzee
Package	100 test ($319)

See all CD4 products

View product citations for antibody GTX74819 on CiteAb

PRODUCT

Summary

The RPA-T4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
FACS	0.5 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test

Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Product Note

Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1 of the extracellular region of the CD4 molecule.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human PHA blasts.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin (PE)

RRID

AB_376477

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD4 molecule , CD4mut

Cellular Localization

Cell membrane

Background

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Database

Gene ID: 920 CD4
UniProt: P01730 CD4

Research Area

DATA IMAGES

GTX74819 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX74819 CD4 antibody [RPA-T4] (PE).
Solid line : Primary antibody
Dashed line : PE mouse IgG1 isotype control
Antibody amount : 0.5 μg