GeneTex
United States (US)

CD4 antibody [W3/25]

GTX76318_1935_IHC-Fr_18121411_729.jpg
GTX76318_1934_IHC-Fr_18121411_121.jpg
GTX76318_1933_IHC-Fr_18121411_905.jpg
GTX76318_1939_IHC-Fr_18121411_907.jpg
GTX76318_1936_IHC-Fr_18121411_243.jpg
GTX76318_1937_IHC-Fr_18121411_819.jpg
GTX76318_1943_IHC-Fr_18121411_837.jpg
GTX76318_1938_IHC-Fr_18121411_146.jpg
GTX76318_1940_IHC-Fr_18121411_621.jpg
GTX76318_1941_IHC-Fr_18121411_145.jpg
GTX76318_1942_IHC-Fr_18121411_851.jpg

Cat No. GTX76318

Host Mouse
Clonality Monoclonal
Clone Name W3/25
Isotype IgG1
Application IHC-P, IHC-Fr, FACS
Reactivity Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS 1/100-1/200

Note :

IHC-P
PLP (periodate-lysine-paraformaldehyde) fixation is recommended for optimal result.

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW

51 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Rat Thymocyte Membrane Glycoproteins

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

W3/25 Antibody , Cd4 Antibody , Cd4 molecule Antibody , p55 Antibody

Background

MHC class II binding protein that may be a candidate gene for collagen-induced arthritis; human CD4 is a receptor required for HIV infection [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
GTX76318_1935_IHC-Fr_18121411_729.jpg

GTX76318 IHC-Fr Image

IHC-Fr stainng of rat lymph node with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 (GTX76318) in green. Merged image is on the right. Medium power

GTX76318_1934_IHC-Fr_18121411_121.jpg

GTX76318 IHC-Fr Image

IHC-Fr stainng of rat lymph node with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 (GTX76318) in green. Merged image is on the right. Low power

GTX76318_1933_IHC-Fr_18121411_905.jpg

GTX76318 IHC-Fr Image

IHC-Fr stainng of rat lymph node with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 (GTX76318) in green. Merged image is on the right. High power

GTX76318_1939_IHC-Fr_18121411_907.jpg

GTX76318 IHC-Fr Image

IHC-Fr staining of rat lymph node with Mouse anti Rat CD172a antibody, red in A and Mouse anti Rat CD4 (GTX76318), green in B. C is the Merged image with nuclei counter-stained blue using DAPI. High power

GTX76318_1936_IHC-Fr_18121411_243.jpg

GTX76318 IHC-Fr Image

IHC-Fr staining of rat lymph node with Mouse anti Rat CD169, clone ED3, red in A and Mouse anti Rat CD4, clone W3/25 (GTX76318), green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power

GTX76318_1937_IHC-Fr_18121411_819.jpg

GTX76318 IHC-Fr Image

IHC-Fr staining of rat lymph node with Mouse anti Rat CD169, clone ED3, red in A and Mouse anti Rat CD4, clone W3/25 (GTX76318), green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power

GTX76318_1943_IHC-Fr_18121411_837.jpg

GTX76318 IHC-Fr Image

IHC-Fr analysis of rat spleen tissue using GTX76318 CD4 antibody [W3/25].

GTX76318_1938_IHC-Fr_18121411_146.jpg

GTX76318 IHC-Fr Image

IHC-Fr staining of rat lymph node with Mouse anti Rat CD68 antibody, red in A and Mouse anti Rat CD4 (GTX76318), green in B. C is the merged image with nuclei counter-stained blue using DAPI. Medium power

GTX76318_1940_IHC-Fr_18121411_621.jpg

GTX76318 IHC-Fr Image

IHC-Fr analysis of rat lymph node tissue using GTX76318 CD4 antibody [W3/25].

GTX76318_1941_IHC-Fr_18121411_145.jpg

GTX76318 IHC-Fr Image

IHC-Fr analysis of rat lymph node tissue using GTX76318 CD4 antibody [W3/25].

GTX76318_1942_IHC-Fr_18121411_851.jpg

GTX76318 IHC-Fr Image

IHC-Fr analysis of rat lymph node tissue using GTX76318 CD4 antibody [W3/25].

REFERENCE
Package List Price ($)
$ 239