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CD4 antibody [YTS177.9] (Low endotoxin, azide free)

Cat. No. GTX44539

Reference 1

Host

 Rat

Clonality

 Monoclonal

Clone Name

 YTS177.9

Isotype

 IgG2a

Application

 FACS, Neutralizing/Inhibition

Reactivity

 Mouse
Package
500 μg ($749)
See all CD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 1/50-1/200
Neutralizing/Inhibition Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Neutralizing/Inhibition
Rat anti Mouse CD4 antibody, clone YTS177.9 also blocks MHC-II dependant T-cell responses in vitro and in vivo and induces tolerance (Qin et al. 1990) and Cobbold et al. 1990).
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse spleen cells

Purification

Ammonium sulphate precipitation

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD4 antigen , L3T4 , Ly-4

Cellular Localization

Cell membrane

Database

Research Area

REFERENCE

Clone Reference

Wise MP et al. J Immunol 1998; 161 (11):1429347 Linked suppression of skin graft rejection can operate through indirect recognition.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 749