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CD4 antibody

Anti-CD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04861
Anti-CD4 antibody used in Western Blot (WB). GTX04861
Anti-CD4 antibody used in Western Blot (WB). GTX04861
Anti-CD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04861
Anti-CD4 antibody used in Western Blot (WB). GTX04861
Anti-CD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04861
Anti-CD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04861
Anti-CD4 antibody used in Western Blot (WB). GTX04861
Anti-CD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04861
Anti-CD4 antibody used in Western Blot (WB). GTX04861
Anti-CD4 antibody used in Flow cytometry (FACS). GTX04861
Anti-CD4 antibody used in Western Blot (WB). GTX04861
Anti-CD4 antibody used in Western Blot (WB). GTX04861
Anti-CD4 antibody used in Flow cytometry (FACS). GTX04861

Cat. No. GTX04861

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Chicken, Pig
Package
100 μl ($399)
See all CD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:300-1:5000
IHC-P 1:200-1:400
FACS 1:20-1:100
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM TBS, 1% BSA, 50% Glycerol

Preservative

0.02% Proclin300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide derived from Human CD4 (cytoplasmic domain, 385-457/457 aa)

Purification

Purified by Protein A

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD4 molecule

Cellular Localization

Cell membrane

Background

MHC class II binding protein that may be a candidate gene for collagen-induced arthritis; human CD4 is a receptor required for HIV infection [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-CD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04861

GTX04861 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX04861 CD4 antibody.
Antigen retrieval : Boiling sodium citrate buffer (pH6.0) for 15min
Dilution : 1:400

Anti-CD4 antibody used in Western Blot (WB). GTX04861

GTX04861 WB Image

WB analysis of various samples using GTX04861 CD4 antibody.
Dilution : 1:1000

Anti-CD4 antibody used in Western Blot (WB). GTX04861

GTX04861 WB Image

WB analysis of mouse spleen tissue lysate using GTX04861 CD4 antibody.
Dilution : 1:300

Anti-CD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04861

GTX04861 IHC-P Image

IHC-P analysis of rat lung tissue using GTX04861 CD4 antibody.
Orange : Primary antibody
Blue : DAPI
Dilution : 1:200

Anti-CD4 antibody used in Western Blot (WB). GTX04861

GTX04861 WB Image

WB analysis of Jurkat whole cell lysate using GTX04861 CD4 antibody.
Dilution : 1:1000

Anti-CD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04861

GTX04861 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX04861 CD4 antibody.
Dilution : 1:400

Anti-CD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04861

GTX04861 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX04861 CD4 antibody.
Antigen retrieval : Boiling sodium citrate buffer (pH6.0) for 15min
Dilution : 1:400

Anti-CD4 antibody used in Western Blot (WB). GTX04861

GTX04861 WB Image

WB analysis of various samples using GTX04861 CD4 antibody.
Lane 1 : CD4 recombinant protein
Lane 2 : Raji whole cell lysate
Dilution : 1:300

Anti-CD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX04861

GTX04861 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX04861 CD4 antibody.
Dilution : 1:200

Anti-CD4 antibody used in Western Blot (WB). GTX04861

GTX04861 WB Image

WB analysis of HL-60 whole cell lysate using GTX04861 CD4 antibody.
Dilution : 1:300

Anti-CD4 antibody used in Flow cytometry (FACS). GTX04861

GTX04861 FACS Image

FACS analysis of mouse spleen cells using GTX04861 CD4 antibody.
Purple : Primary antibody
Dilution : 1:50

Anti-CD4 antibody used in Western Blot (WB). GTX04861

GTX04861 WB Image

WB analysis of U937 whole cell lysate using GTX04861 CD4 antibody.
Dilution : 1:300

Anti-CD4 antibody used in Western Blot (WB). GTX04861

GTX04861 WB Image

WB analysis of mouse lymph node tissue lysate using GTX04861 CD4 antibody.
Dilution : 1:300

Anti-CD4 antibody used in Flow cytometry (FACS). GTX04861

GTX04861 FACS Image

FACS analysis of 2% PFA-fixed HL-60 cells using GTX04861 CD4 antibody.
Green : Primary antibody
Orange : Isotype control
Light blue : Secondary antibody only
Dark blue : Unlabelled sample
Permeabilization : 0.1% PBST
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399