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CD40 antibody [5C3]

Anti-CD40 antibody [5C3] used in Flow cytometry (FACS). GTX00875
Anti-CD40 antibody [5C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00875

Cat. No. GTX00875

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5C3

Isotype

 IgG1

Application

 ICC/IF, IHC-Fr, FACS, Activation

Reactivity

 Human
Package
100 μg ($399)
See all CD40 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100
IHC-Fr 1:100
FACS Assay dependent
Activation 20 μg /ml

Note :

IHC-Fr
Recommed using acetone-fixed or zinc-fixed section.

Activation
Stimulation of proliferation of B cell and dendritic cells and enhancement of proinflamentory cytokine production in human monocytes, such as TNF-alpha, IL-6 and IL-8.
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant extracellular domain of CD40

Purification

Purified by salting-out and ion-exchange chromatography
From culture supernant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD40 molecule , Bp50 , CD40 , CDW40 , TNFRSF5 , p50

Cellular Localization

Cell membrane,Secreted

Background

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-CD40 antibody [5C3] used in Flow cytometry (FACS). GTX00875

GTX00875 FACS Image

FACS analysis of monocyte-derived dendric cells from healthy adult donor using GTX00875 CD40 antibody [5C3].
Solid line : Primary antibody
Broken line : Isotype control

Anti-CD40 antibody [5C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00875

GTX00875 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX00875 CD40 antibody [5C3].
Fixation : 4% PFA
Permeabilization : 0.25% Triton X-100
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399