CD40 antibody [FGK4.5/ FGK45] (APC)

Cat. No. GTX01483-07

Host	Rat
Clonality	Monoclonal
Clone Name	FGK4.5/ FGK45
Isotype	IgG2a
Application	FACS
Reactivity	Mouse
Package	100 μg ($319)

See all CD40 products

View product citations for antibody GTX01483-07 on CiteAb

PRODUCT

Summary

The FGK45 antibody reacts with mouse CD40, a 40-50 kDa glycoprotein member of the tumor necrosis factor receptor (TNFR) family. CD40 is constitutively expressed by antigen presenting cells, including dendritic cells, B cells, and macrophages. The interaction of CD40 and its ligand CD154, expressed on activated T cells, plays an essential role in mediating a broad range of immune and inflammatory responses including T cell dependent immunoglobulin class switching, germinal center formation, and the development of memory B cells. The FGK45 clone is reported to be functionally useful for both in vitro and in vivo activation of CD40 expressing antigen presenting cells. The FGK45 antibody reacts with mouse CD40, a 40-50 kDa glycoprotein member of the tumor necrosis factor receptor (TNFR) family.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
FACS	Assay dependent

Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cell membrane,Secreted

Database

Gene ID: 21939 Cd40
UniProt: P27512 Cd40

Research Area

DATA IMAGES

GTX01483-07 FACS Image

FACS analysis of mouse C57Bl/6 splenocytes using GTX01483-07 CD40 antibody [FGK45] (APC).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.25 μg (5 μl)