APPLICATION
Application Note
For flow cytometry: Use 10ul of the suggested working dilution (20μg/ml) to label 10^6 cells in 100ul. For immunohistochemistry: * This product requires protein digestion pre-treatment of paraffin-embedded sections prior to staining eg. pronase or trypsin. Optimal dilutions/concentrations should be determined by the researcher.
Calculated MW
Positive Control
Tonsil
Product Note
Recognises the human CD40 cell surface antigen, a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.20 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Purification
Purified IgG
Conjugation
Unconjugated
RRID
AB_376650
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD40 molecule , Bp50 , CDW40 , TNFRSF5 , p50
Background
This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]
Database
Research Area
REFERENCE
There are currently no references for CD40 antibody [LOB7/6] (GTX75202). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD40 antibody [LOB7/6] (GTX75202). Be the first to share your experience with this product.
