CD40L / CD154 antibody [5C8] (APC)

Cat. No. GTX01484-07

Host	Mouse
Clonality	Monoclonal
Clone Name	5C8
Isotype	IgG2a
Application	FACS
Reactivity	Human, Cynomolgus monkey, Rhesus Monkey
Package	100 test ($409)

See all CD40L / CD154 products

View product citations for antibody GTX01484-07 on CiteAb

PRODUCT

Summary

The 5C8 antibody reacts with human CD154 which is also known as CD40L (CD40 ligand). CD154 is a 39kDa member of the TNF-α superfamily and is predominantly expressed on activated T cells but is also expressed on NK cells, mast cells, basophils, and eosinophils. CD154 binds to CD40, a molecule that is expressed on antigen presenting cells including B cells, monocytes/macrophages, and dendritic cells. The interaction of CD154 and CD40 regulates the activation of antigen presenting cells and is critical for B cell costimulation. The 5C8 antibody has been reported to block CD40L-CD40 interactions.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
FACS	0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test

Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD40 ligand , CD154 , CD40L , CD40LG , HIGM1 , IGM , IMD3 , TBAM , TNFSF5 , TRAP , gp39 , hCD40L

Cellular Localization

Cell membrane,Secreted

Background

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]

Database

Gene ID: 959 CD40LG
UniProt: P29965 CD40LG

Research Area

DATA IMAGES

GTX01484-07 FACS Image

FACS analysis of human PBMCs (with PMA and Ionomycin stimulation for 5 hrs) using GTX01484-07 CD40L / CD154 antibody [5C8] (APC).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.25 μg (5 μl)