CD40L / CD154 antibody [MR1] (Biotin)

Cat. No. GTX01485-02

Host	Armenian Hamster
Clonality	Monoclonal
Clone Name	MR1
Isotype	IgG
Application	FACS
Reactivity	Mouse
Package	100 μg ($169)

See all CD40L / CD154 products

View product citations for antibody GTX01485-02 on CiteAb

PRODUCT

Summary

The MR1 antibody is specific for mouse CD154, a 39 kD glycoprotein that is primarily expressed on activated T cells. CD154 (CD40 ligand, gp39) binds to CD40 expressed on antigen presenting cells and mediates important costimulatory functions between these cells. Signals generated via cognate interactions between CD154-CD40 on activated T and B cells are required for T-dependent B cell responses. The MR1 antibody is reported to block interaction of CD154 with CD40.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
FACS	Assay dependent
Note : FACS For detection of CD154 on in vitro activated T cells, it is recommended that T cells be enriched using a depletion strategy prior to stimulation.

Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Biotin

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cell membrane,Cell surface,Secreted

Database

Gene ID: 21947 Cd40lg
UniProt: P27548 Cd40lg

Research Area

DATA IMAGES

GTX01485-02 FACS Image

FACS analysis of mouse C57Bl/6 T cells (enriched from total splenocytes, with PMA and Ionomycin stimulation for 6 hrs) using GTX01485-02 CD40L / CD154 antibody [MR1] (Biotin).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.25 μg (5 μl)