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CD40L / CD154 antibody, N-term

Anti-CD40L / CD154 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81854
Anti-CD40L / CD154 antibody, N-term used in Western Blot (WB). GTX81854
Anti-CD40L / CD154 antibody, N-term used in Western Blot (WB). GTX81854
Anti-CD40L / CD154 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81854
Anti-CD40L / CD154 antibody, N-term used in Flow cytometry (FACS). GTX81854

Cat. No. GTX81854

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all CD40L / CD154 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:50
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human TRAP.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

RRID

AB_11166062

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD40 ligand , CD154 , CD40L , HIGM1 , IGM , IMD3 , T-BAM , TNFSF5 , TRAP , gp39 , hCD40L

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD40L / CD154 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81854

GTX81854 ICC/IF Image

ICC/IF analysis of NCI-H460 cells using GTX81854 CD40L / CD154 antibody, N-term.
Green : CD40L / CD154
Blue : DAPI

Anti-CD40L / CD154 antibody, N-term used in Western Blot (WB). GTX81854

GTX81854 WB Image

WB analysis of various samples using GTX81854 CD40L / CD154 antibody, N-term.
Lane 1: Jurkat whole cell lysate
Lane 2: THP-1 whole cell lysate
Loading : 20 μg per lane
Dilution : 1:1000

Anti-CD40L / CD154 antibody, N-term used in Western Blot (WB). GTX81854

GTX81854 WB Image

WB analysis of NCI-H460 cell lysate (35ug/lane) using GTX81854 CD40L / CD154 antibody, N-term.

Anti-CD40L / CD154 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81854

GTX81854 IHC-P Image

IHC-P analysis of human kidney tissue using GTX81854 CD40L / CD154 antibody, N-term.

Anti-CD40L / CD154 antibody, N-term used in Flow cytometry (FACS). GTX81854

GTX81854 FACS Image

FACS analysis of NCI-H460 cells using GTX81854 CD40L / CD154 antibody, N-term.
Top histogram : negative control
Bottom histogram : NCI-H460 cells

REFERENCE

Application Reference

Miura S et al. J Biol Chem 2020; 295 (38) : 13194-13201 Amyloid precursor protein 770 is specifically expressed and released from platelets.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399