GeneTex
United States (US)

CD40L / CD154 antibody

Cat No. GTX10507

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, FACS
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/ml
FACS Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS containing carbohydrates

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

purified recombinant human CD40 ligand C-terminal extracellular domain, expressed in E. coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd40 Ligand,Cd154,Cd40L,Higm1,Igm,Imd3,T-Bam,Tnfsf5,Trap,Gp39,Hcd40L,Cd40Lg

Background

CD40 ligand (also known as CD154, TRAP, or gp39) is a 261 amino acid type II transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) superfamily. CD40L is expressed predominantly on activated CD4+ T lymphocytes. It is also found in other cell types (NK cells, mast cells, basophils, and eosinophils). Human and mouse CD40L share approximately 78% sequence identity. The receptor for CD40L is CD40. CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family, is expressed on B lymphocytes, monocytes, dendritic cells, and thymic epithelium. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions central to the control of thymus-dependent humoral immunity and may be critical in the development of cell-mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. The interaction of CD40 and CD40L also plays important roles in monocyte activation and dendritic cell maturation. Activation of CD40 inhibits the growth of certain B cell lymphomas and induces the death of transformed cells of mesenchymal or epithelial origin.

Database

Research Area

Package List Price ($)
$ 319