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CD41 antibody [N2C1], Internal

Anti-CD41 antibody [N2C1], Internal used in Western Blot (WB). GTX113758
Anti-CD41 antibody [N2C1], Internal used in Western Blot (WB). GTX113758
Anti-CD41 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113758

Cat. No. GTX113758

Reference 9
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CD41 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Rabbit, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CD41. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617930

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit alpha 2b , BDPLT16 , BDPLT2 , CD41 , CD41B , GP2B , GPIIb , GT , GTA , HPA3 , PPP1R93

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

ITGA2B encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibronectin receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD41 antibody [N2C1], Internal used in Western Blot (WB). GTX113758

GTX113758 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD41 antibody [N2C1], Internal (GTX113758) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD41 antibody [N2C1], Internal used in Western Blot (WB). GTX113758

GTX113758 WB Image

Sample (30 μg of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX113758 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD41 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113758

GTX113758 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using CD41(GTX113758) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Ye Y et al. Front Pharmacol 2023; 14 : 1255069 Luteolin inhibits GPVI-mediated platelet activation, oxidative stress, and thrombosis.
Cassidy Delaney et al. Am J Physiol Lung Cell Mol Physiol 2021; 320 (3) : L413-L421 Platelet activation contributes to hypoxia-induced inflammation.
Application : IHC-P
Reactivity : Human
Youn DH et al. Sci Rep 2021; 11 (1) : 16512 Mitochondrial dysfunction associated with autophagy and mitophagy in cerebrospinal fluid cells of patients with delayed cerebral ischemia following subarachnoid hemorrhage.
Application : ICC/IF
Reactivity : Human
Davizon-Castillo P et al. Physiol Rep 2020; 8 (5) : e14386 Platelet activation in experimental murine neonatal pulmonary hypertension.
Application : IHC-Fr
Reactivity : Mouse
Takahashi A et al. J Investig Clin Dent 2019; : e12458 Distribution of platelets, transforming growth factor-帣1, platelet-derived growth factor-BB, vascular endothelial growth factor and matrix metalloprotease-9 in advanced platelet-rich fibrin and concentrated growth factor matrices.
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Submit a Reference

REVIEW

CD41 antibody [N2C1], Internal Cat. No. GTX113758

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-CD41 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX113758
Date : Anonymous submitted on 14-May-2019
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Human platelet-rich fibrin
Blocking : Block Ace in 0.1% Tween 20-containing PBS, 25°C, 1Hr
Fixation : 10% buffered formaline
Permeabilization /Antigen retrieval : LAB solution
Primary Antibody : 1:400, 4°C, 20Hr
Submit a Review
Application Reference
Ye Y et al. Front Pharmacol 2023; 14:1255069 Luteolin inhibits GPVI-mediated platelet activation, oxidative stress, and thrombosis.
Cassidy Delaney et al. Am J Physiol Lung Cell Mol Physiol 2021; 320 (3):L413-L421 Platelet activation contributes to hypoxia-induced inflammation.
Application : IHC-P
Reactivity : Human
Youn DH et al. Sci Rep 2021; 11 (1):16512 Mitochondrial dysfunction associated with autophagy and mitophagy in cerebrospinal fluid cells of patients with delayed cerebral ischemia following subarachnoid hemorrhage.
Application : ICC/IF
Reactivity : Human
Davizon-Castillo P et al. Physiol Rep 2020; 8 (5):e14386 Platelet activation in experimental murine neonatal pulmonary hypertension.
Application : IHC-Fr
Reactivity : Mouse
Takahashi A et al. J Investig Clin Dent 2019;:e12458 Distribution of platelets, transforming growth factor-帣1, platelet-derived growth factor-BB, vascular endothelial growth factor and matrix metalloprotease-9 in advanced platelet-rich fibrin and concentrated growth factor matrices.
Bill Cheng et al. RSC Adv 2018; 8 (28):15471-15479 The formulation and characterization of 3D printed grafts as vascular access for potential use in hemodialysis.
Application : ICC/IF
Reactivity : Human
Cheng B. et al. RSC Adv. 2018; 8:15471-15479 The formulation and characterization of 3D printed grafts as vascular access for potential use in hemodialysis.
Application : ICC/IF
Reactivity : Human
Fernando MR et al. PLoS One 2017; 12 (8):e0183915 New evidence that a large proportion of human blood plasma cell-free DNA is localized in exosomes.
Application : WB
Reactivity : Human
Cheng B et al. Adv Healthc Mater 2016; Biomimicking Platelet-Monocyte Interactions as a Novel Targeting Strategy for Heart Healing.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169