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CD42b antibody [AK2] (Low endotoxin, azide free)

Anti-CD42b antibody [AK2] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX76458

Cat. No. GTX76458

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AK2

Isotype

 IgG1

Application

 FACS, IP, ELISA, Neutralizing/Inhibition

Reactivity

 Human
Package
250 μg ($399)
See all CD42b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 1/50-1/100
IP Assay dependent
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.

Neutralizing/Inhibition
CD42b is expressed by platelets and megakaryocytes. Clone AK2 has been reported to block the binding of von Willebrand Factor (VWF) to platelets.
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Protein G purified
From tissue culture supernatant

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

RRID

AB_377364

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Glycoprotein Ib Platelet Subunit Alpha , Bdplt1 , Bdplt3 , Bss , Cd42B , Cd42B-Alpha , Dbplt3 , Gp1B , Gpiba , Gpibalpha , Vwdp , Gp1Ba

Background

Glycoprotein Ib (GP Ib) is a platelet surface membrane glycoprotein composed of a heterodimer, an alpha chain and a beta chain, that is linked by disulfide bonds. The Gp Ib functions as a receptor for von Willebrand factor (VWF). The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX and platelet glycoprotein V. The binding of the GP Ib-IX-V complex to VWF facilitates initial platelet adhesion to vascular subendothelium after vascular injury, and also initiates signaling events within the platelet that lead to enhanced platelet activation, thrombosis, and hemostasis. This gene encodes the alpha subunit. Mutations in this gene result in Bernard-Soulier syndromes and platelet-type von Willebrand disease. The coding region of this gene is known to contain a polymophic variable number tandem repeat (VNTR) domain that is associated with susceptibility to nonarteritic anterior ischemic optic neuropathy. [provided by RefSeq, Oct 2013]

Research Area

DATA IMAGES

Anti-CD42b antibody [AK2] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX76458

GTX76458 FACS Image

FACS analysis of human peripheral blood platelets using GTX76458 CD42b antibody [AK2] (Low endotoxin, azide free).

REFERENCE

Clone Reference

Zilberman-Rudenko J et al. Cardiovasc Eng Technol 2018; (Epub) Effect of Pneumatic Tubing System Transport on Platelet Apheresis Units.
Tsubota Y et al. J Leukoc Biol 2014; 95 (1):191-5 Novel ex vivo culture method for human monocytes uses shear flow to prevent total loss of transendothelial diapedesis function.
Alcaide P et al. Infect Immun 2010; 78 (3):1260-8 Mucin AgC10 from Trypanosoma cruzi Interferes with L-selectin-mediated monocyte adhesion.
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations