APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
Not tested in other applications.
Calculated MW
Product Note
Epitope of MAb Bra7G is clearly different from that of MAb DF-T1, called b as opposed to a for DF-T1.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Immature pluripotent human leukemia K562 cells
Purification
PEG precipitation
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD43 , GALGP , GPL115 , LSN , SPN , sialophorin
Cellular Localization
Membrane
Background
This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017]
Database
Research Area
DATA IMAGES
![Anti-CD43 antibody [Bra7G] used in IHC (Paraffin sections) (IHC-P). GTX34510](/templates/default/js/lightbox/images/loading.gif "Anti-CD43 antibody [Bra7G] used in IHC (Paraffin sections) (IHC-P). GTX34510")
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GTX34510 IHC-P Image
IHC-P analysis of human spleen tissue using GTX34510 CD43 antibody [Bra7G].
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GTX34510 ICC/IF Image
ICC/IF analysis of K562 cells using GTX34510 CD43 antibody [Bra7G].
Green : Primary antibody
Red : nucleus
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REFERENCE
There are currently no references for CD43 antibody [Bra7G] (GTX34510). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD43 antibody [Bra7G] (GTX34510). Be the first to share your experience with this product.
![Anti-CD43 antibody [Bra7G] used in IHC (Paraffin sections) (IHC-P). GTX34510](/upload/website/prouct_img/small/GTX34510/GTX34510_20200115_IHC-P_1160_w_23060801_135.webp "Anti-CD43 antibody [Bra7G] used in IHC (Paraffin sections) (IHC-P). GTX34510")
![Anti-CD43 antibody [Bra7G] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34510](/upload/website/prouct_img/small/GTX34510/GTX34510_20200115_ICC-IF_1510_w_23060801_249.webp "Anti-CD43 antibody [Bra7G] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34510")
![Anti-CD43 antibody [Bra7G] used in IHC (Paraffin sections) (IHC-P). GTX34510](/upload/website/prouct_img/normal/GTX34510/GTX34510_20200115_IHC-P_1160_w_23060801_135.webp "Anti-CD43 antibody [Bra7G] used in IHC (Paraffin sections) (IHC-P). GTX34510")
![Anti-CD43 antibody [Bra7G] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34510](/upload/website/prouct_img/normal/GTX34510/GTX34510_20200115_ICC-IF_1510_w_23060801_249.webp "Anti-CD43 antibody [Bra7G] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34510")