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CD43 antibody [Bra7G]

Anti-CD43 antibody [Bra7G] used in IHC (Paraffin sections) (IHC-P). GTX34510

Cat No. GTX34510

Host

Mouse

Clonality

Monoclonal

Clone Name

Bra7G

Isotype

IgM

Application

ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Digest sections with trypsin at 1mg/ml PBS, 15 min at room temp

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Specificity/Sensitivity

It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop V). Epitope of MAb Bra7G is clearly different from that of MAb DF-T1, called b as opposed to a for DF-T1. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Immature pluripotent human leukemia K562 cells

Purification

Ab purified from Bioreactor Concentrate by PEG precipitation

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Sialophorin , Cd43 , Galgp , Gpl115 , Lsn , Spn

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-CD43 antibody [Bra7G] used in IHC (Paraffin sections) (IHC-P). GTX34510

GTX34510 IHC-P Image

Formalin-fixed, paraffin-embedded human Spleen stained with CD43 Monoclonal Antibody (Bra7G).

REFERENCE

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REVIEW

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Package List Price ($)
$ 429