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CD43 antibody [Bra7G]

Anti-CD43 antibody [Bra7G] used in IHC (Paraffin sections) (IHC-P). GTX34510
Anti-CD43 antibody [Bra7G] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34510

Cat No. GTX34510

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 Bra7G

Isotype

 IgM

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μg ($339)
See all CD43 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Specificity/Sensitivity

Epitope of MAb Bra7G is clearly different from that of MAb DF-T1, called b as opposed to a for DF-T1.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Immature pluripotent human leukemia K562 cells

Purification

PEG precipitation

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD43 , GALGP , GPL115 , LSN , SPN , sialophorin

Cellular Localization

Membrane

Background

This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES

Anti-CD43 antibody [Bra7G] used in IHC (Paraffin sections) (IHC-P). GTX34510

GTX34510 IHC-P Image

IHC-P analysis of human spleen tissue using GTX34510 CD43 antibody [Bra7G].

Anti-CD43 antibody [Bra7G] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34510

GTX34510 ICC/IF Image

ICC/IF analysis of K562 cells using GTX34510 CD43 antibody [Bra7G].
Green : Primary antibody
Red : nucleus

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339