APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1-2ug/ml |
| 0.5-1ug/ml for 30 minutes at RT |
|
Note :
IHC-P
Digest sections with trypsin at 1mg/ml PBS, 15 min at room temp
|
Not tested in other applications.
Calculated MW
Specificity/Sensitivity
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop V). Epitope of MAb Bra7G is clearly different from that of MAb DF-T1, called b as opposed to a for DF-T1. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
PROPERTIES
Form
Liquid
Buffer
10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml
Antigen Species
Human
Immunogen
Immature pluripotent human leukemia K562 cells
Purification
Ab purified from Bioreactor Concentrate by PEG precipitation
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Sialophorin , Cd43 , Galgp , Gpl115 , Lsn , Spn
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response. [provided by RefSeq, Sep 2011]
Database
Research Area
DATA IMAGES
![Anti-CD43 antibody [Bra7G] used in IHC (Paraffin sections) (IHC-P). GTX34510](/upload/website/prouct_img/middle/GTX34510/GTX34510_20170712_IHC-P_18121410_336.jpg "Anti-CD43 antibody [Bra7G] used in IHC (Paraffin sections) (IHC-P). GTX34510")
|
GTX34510 IHC-P Image
Formalin-fixed, paraffin-embedded human Spleen stained with CD43 Monoclonal Antibody (Bra7G).
|
REFERENCE
There are currently no references for CD43 antibody [Bra7G] (GTX34510). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD43 antibody [Bra7G] (GTX34510). Be the first to share your experience with this product.
![Anti-CD43 antibody [Bra7G] used in IHC (Paraffin sections) (IHC-P). GTX34510](/upload/website/prouct_img/small/GTX34510/GTX34510_20170712_IHC-P_18121410_336.jpg "Anti-CD43 antibody [Bra7G] used in IHC (Paraffin sections) (IHC-P). GTX34510")
![Anti-CD43 antibody [Bra7G] used in IHC (Paraffin sections) (IHC-P). GTX34510](/upload/website/prouct_img/normal/GTX34510/GTX34510_20170712_IHC-P_18121410_336.jpg "Anti-CD43 antibody [Bra7G] used in IHC (Paraffin sections) (IHC-P). GTX34510")
