*Optimal dilutions/concentrations should be determined by the researcher.
|1-2μg/ml for 30 minutes at RT
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.
Epitope of MAb Bra7G is clearly different from that of MAb DF-T1, called b as opposed to a for DF-T1.
10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Myeloblastic KG1 cells
Protein A/G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
CD43 , GALGP , GPL115 , LSN , SPN , sialophorin
This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017]
GTX34513 WB Image
WB analysis of K562 cell lysate using GTX34513 CD43 antibody [DF-T1].
GTX34513 ICC/IF Image
ICC/IF analysis of K562 cells using GTX34513 CD43 antibody [DF-T1].
Green : Primary antibody
Red : nucleus
GTX34513 FACS Image
FACS analysis of PFA-fixed K562 cells using GTX34513 CD43 antibody [DF-T1].
Blue : Primary antibody
Red : Isotype control
GTX34513 FACS Image
FACS analysis of human PBMCs using GTX34513 CD43 antibody [DF-T1].
Red : Primary antibody
Green : Isotype control
GTX34513 IHC-P Image
IHC-P analysis of human spleen tissue using GTX34513 CD43 antibody [DF-T1].