GeneTex

  • Country / Location Selection

United States (US)

CD43 antibody [DFT-1] (FITC)

Anti-CD43 antibody [DFT-1] (FITC) used in Flow cytometry (FACS). GTX76309

Cat. No. GTX76309

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 DFT-1

Isotype

 IgG1

Application

 FACS

Reactivity

 Human
Package
50 μg ($329)
See all CD43 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Neat

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Product Note

This antibody binds to both glycoforms of human CD43 (Kyoizumi et al. 2004)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KG-1 cells.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

RRID

AB_377277

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sialophorin , CD43 , GALGP , GPL115 , LSN

Cellular Localization

Membrane

Background

This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES

Anti-CD43 antibody [DFT-1] (FITC) used in Flow cytometry (FACS). GTX76309

GTX76309 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX76309 CD43 antibody [DFT-1] (FITC).

REFERENCE

Clone Reference

Kyoizumi S et al. J Immunol. 2004; 172 (12):7246-53 Expression characteristics and stimulatory functions of CD43 in human CD4+ memory T cells: analysis using a monoclonal antibody to CD43 that has a novel lineage specificity.
Application : FACS
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for CD43 antibody [DFT-1] (FITC) (GTX76309). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329