GeneTex
United States (US)

CD43 antibody [SPM503]

GTX34507_20170712_FACS_18121410_942.jpg
GTX34507_20170712_IHC-P_18121410_257.jpg

Cat No. GTX34507

Host Mouse
Clonality Monoclonal
Clone Name SPM503
Isotype IgG1
Application IHC-P, FACS
Reactivity Human
APPLICATION

Application Note

IHC-P:0.5-1ug/ml for 30 minutes at RT, FACS:0.5-1ug/million cells in 0.1ml.

Calculated MW

40 kDa. ( Note )

Specificity/Sensitivity

It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

Myeloblastic KG1 cells

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sialophorin,Cd43,Galgp,Gpl115,Lsn,Spn

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES
GTX34507_20170712_FACS_18121410_942.jpg

GTX34507 FACS Image

Flow Cytometry of PBMC using CD43 Monoclonal Antibody (SPM503) (red) or isotype control (green)

GTX34507_20170712_IHC-P_18121410_257.jpg

GTX34507 IHC-P Image

Formalin-fixed, paraffin-embedded human Spleen stained with CD43 Monoclonal Antibody (SPM503).

Package List Price ($)
$ 329