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CD43 antibody [SPM503]

Anti-CD43 antibody [SPM503] used in Flow cytometry (FACS). GTX34507
Anti-CD43 antibody [SPM503] used in IHC (Paraffin sections) (IHC-P). GTX34507

Cat No. GTX34507

Host

Mouse

Clonality

Monoclonal

Clone Name

SPM503

Isotype

IgG1

Application

ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT
FACS Assay dependent

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Specificity/Sensitivity

It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Immunogen

Myeloblastic KG1 cells

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Sialophorin , Cd43 , Galgp , Gpl115 , Lsn , Spn

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-CD43 antibody [SPM503] used in Flow cytometry (FACS). GTX34507

GTX34507 FACS Image

Flow Cytometry of PBMC using CD43 Monoclonal Antibody (SPM503) (red) or isotype control (green)

Anti-CD43 antibody [SPM503] used in IHC (Paraffin sections) (IHC-P). GTX34507

GTX34507 IHC-P Image

Formalin-fixed, paraffin-embedded human Spleen stained with CD43 Monoclonal Antibody (SPM503).

REFERENCE

There are currently no references for CD43 antibody [SPM503] (GTX34507). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CD43 antibody [SPM503] (GTX34507). Be the first to share your experience with this product.
Package List Price ($)
$ 429