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CD43 antibody [SPN/1094]

Anti-CD43 antibody [SPN/1094] used in IHC (Paraffin sections) (IHC-P). GTX34511
Anti-CD43 antibody [SPN/1094] used in IHC (Paraffin sections) (IHC-P). GTX34511
Anti-CD43 antibody [SPN/1094] used in Western Blot (WB). GTX34511
Anti-CD43 antibody [SPN/1094] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34511

Cat No. GTX34511

Host

Mouse

Clonality

Monoclonal

Clone Name

SPN/1094

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant full-length human SPN protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD43,GALGP,GPL115,LSN,SPN,sialophorin

Cellular Localization

Membrane

Background

This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES

Anti-CD43 antibody [SPN/1094] used in IHC (Paraffin sections) (IHC-P). GTX34511

GTX34511 IHC-P Image

IHC-P analysis of human lymphoma tissue using GTX34511 CD43 antibody [SPN/1094].

Anti-CD43 antibody [SPN/1094] used in IHC (Paraffin sections) (IHC-P). GTX34511

GTX34511 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34511 CD43 antibody [SPN/1094].

Anti-CD43 antibody [SPN/1094] used in Western Blot (WB). GTX34511

GTX34511 WB Image

WB analysis of K562 cell lysate using GTX34511 CD43 antibody [SPN/1094].

Anti-CD43 antibody [SPN/1094] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34511

GTX34511 ICC/IF Image

ICC/IF analysis of K562 cells using GTX34511 CD43 antibody [SPN/1094].
Green : Primary antibody
Red : nucleus

REFERENCE

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REVIEW

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Package List Price ($)
$ 339