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CD43 antibody [SPN/1094]

Anti-CD43 antibody [SPN/1094] used in IHC (Paraffin sections) (IHC-P). GTX34511
Anti-CD43 antibody [SPN/1094] used in IHC (Paraffin sections) (IHC-P). GTX34511

Cat No. GTX34511

Host Mouse
Clonality Monoclonal
Clone Name SPN/1094
Isotype IgG1
Application ICC/IF, IHC-P
Reactivity Human
Package
100 μg ($429)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Specificity/Sensitivity

It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant human SPN protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sialophorin , Cd43 , Galgp , Gpl115 , Lsn , Spn

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, this protein is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune response. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES
Anti-CD43 antibody [SPN/1094] used in IHC (Paraffin sections) (IHC-P). GTX34511

GTX34511 IHC-P Image

Formalin-fixed, paraffin-embedded human Lymphoma stained with CD43 Monoclonal Antibody (SPN/1094).

Anti-CD43 antibody [SPN/1094] used in IHC (Paraffin sections) (IHC-P). GTX34511

GTX34511 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with CD43 Monoclonal Antibody (SPN/1094).

REFERENCE
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REVIEW
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Package List Price ($)
$ 429