CD43 antibody [SPN/2049R]

Cat. No. GTX02714

Host	Rabbit
Clonality	Monoclonal
Clone Name	SPN/2049R
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μg ($529)

See all CD43 products

Datasheet

View product citations for antibody GTX02714 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2 μg/ml
ICC/IF	1-2 μg/ml
IHC-P	1-2 μg/ml
FACS	1-2 μg/million cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Observed MW

130-140 kDa. The observed M.W. is based on the following publication. PMID: 16339541

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human CD43 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sialophorin , CD43 , GALGP , GPL115 , LSN

Cellular Localization

Membrane

Background

This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017]

Database

Gene ID: 6693 SPN
UniProt: P16150 SPN

Research Area

DATA IMAGES

GTX02714 WB Image

WB analysis of K562 whole cell lysate using GTX02714 CD43 antibody [SPN/2049R].

GTX02714 FACS Image

FACS analysis of PFA-fixed K562 cells using GTX02714 CD43 antibody [SPN/2049R].
Blue : Primary antibody
Red : Isotype control

GTX02714 ICC/IF Image

ICC/IF analysis of K562 cells using GTX02714 CD43 antibody [SPN/2049R].
Green : Primary antibody
Red : nuclear counterstain

GTX02714 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX02714 CD43 antibody [SPN/2049R].

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 529

Qty