GeneTex
United States (US)

CD44 antibody [1M7.8.1]

GTX15834_334_ICC-IF_19011519_685.jpg
GTX15834_123_FACS_19011519_586.jpg
GTX15834_336_ICC-IF_19011519_600.jpg
GTX15834_335_ICC-IF_19011519_455.jpg

Cat No. GTX15834

Host Rat
Clonality Monoclonal
Clone Name 1M7.8.1
Isotype IgG2b
Application WB, ICC/IF, FACS, IP, Inhibition assay
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:10-1:100
FACS Assay dependent
IP Assay dependent
Inhibition assay Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Specificity/Sensitivity

This antibody detects a standard 85-kDa isoform of CD44 and a number of high molecular mass variant isoforms.
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse 80-95 kD lymphocyte surface glycoprotein H-CAM (CD44).

Purification

Protein G purified

Purity

This product is a Low Endotoxin formulation.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX15834_334_ICC-IF_19011519_685.jpg

GTX15834 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX15834 CD44 antibody [1M7.8.1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4°C

GTX15834_123_FACS_19011519_586.jpg

GTX15834 FACS Image

FACS analysis of Hela cells using GTX15834 CD44 antibody [1M7.8.1] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

GTX15834_336_ICC-IF_19011519_600.jpg

GTX15834 ICC/IF Image

ICC/IF analysis of Hela cells using GTX15834 CD44 antibody [1M7.8.1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4°C

GTX15834_335_ICC-IF_19011519_455.jpg

GTX15834 ICC/IF Image

ICC/IF analysis of BAF-3 cells using GTX15834 CD44 antibody [1M7.8.1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4°C

REFERENCE
SDS
PBS.pdf
Package List Price ($)
$ 319