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CD44 antibody [1M7.8.1]

Anti-CD44 antibody [1M7.8.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15834
Anti-CD44 antibody [1M7.8.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15834
Anti-CD44 antibody [1M7.8.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15834
Anti-CD44 antibody [1M7.8.1] used in Flow cytometry (FACS). GTX15834

Cat. No. GTX15834

Reference 1

Host

 Rat

Clonality

 Monoclonal

Clone Name

 1M7.8.1

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS, IP, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rat
Package
250 μg ($399)
See all CD44 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:10-1:100
FACS Assay dependent
IP Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Product Note

This antibody detects a standard 85-kDa isoform of CD44 and a number of high molecular mass variant isoforms.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse 80-95 kD lymphocyte surface glycoprotein H-CAM (CD44).

Purification

Protein G purified

Purity

This product is a Low Endotoxin formulation.

Conjugation

Unconjugated

RRID

AB_2887524

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD44 antigen , AU023126 , AW121933 , AW146109 , HERMES , Ly-24 , Pgp-1

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-CD44 antibody [1M7.8.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15834

GTX15834 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX15834 CD44 antibody [1M7.8.1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

Anti-CD44 antibody [1M7.8.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15834

GTX15834 ICC/IF Image

ICC/IF analysis of BAF-3 cells using GTX15834 CD44 antibody [1M7.8.1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

Anti-CD44 antibody [1M7.8.1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15834

GTX15834 ICC/IF Image

ICC/IF analysis of Hela cells using GTX15834 CD44 antibody [1M7.8.1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

Anti-CD44 antibody [1M7.8.1] used in Flow cytometry (FACS). GTX15834

GTX15834 FACS Image

FACS analysis of Hela cells using GTX15834 CD44 antibody [1M7.8.1] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

REFERENCE

Application Reference

Kamioka Y et al. Cancer Sci 2017; 108 (2) : 226-235 Intravital Forster resonance energy transfer imaging reveals osteopontin-mediated polymorphonuclear leukocyte activation by tumor cell emboli.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations