APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/100-1/200 |
| Assay dependent |
| 1/25-1/100 |
| Assay dependent |
|
Note :
IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody recognizes an epitope that is common to all CD44 isoforms.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Purification
Protein A purified
From tissue culture supernatant
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-CD44 antibody [Bu52] used in IHC (Paraffin sections) (IHC-P). GTX44555](/templates/default/js/lightbox/images/loading.gif "Anti-CD44 antibody [Bu52] used in IHC (Paraffin sections) (IHC-P). GTX44555")
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GTX44555 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX44555 CD44 antibody [Bu52].
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REFERENCE
There are currently no references for CD44 antibody [Bu52] (GTX44555). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD44 antibody [Bu52] (GTX44555). Be the first to share your experience with this product.
![Anti-CD44 antibody [Bu52] used in IHC (Paraffin sections) (IHC-P). GTX44555](/upload/website/prouct_img/small/GTX44555/GTX44555_2172_IHC-P_w_23051501_444.webp "Anti-CD44 antibody [Bu52] used in IHC (Paraffin sections) (IHC-P). GTX44555")
![Anti-CD44 antibody [Bu52] used in IHC (Paraffin sections) (IHC-P). GTX44555](/upload/website/prouct_img/normal/GTX44555/GTX44555_2172_IHC-P_w_23051501_444.webp "Anti-CD44 antibody [Bu52] used in IHC (Paraffin sections) (IHC-P). GTX44555")