APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
1/50-1/100 |
Assay dependent |
1/10-1/20 |
Note :
FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody recognizes an epitope on human CD44 outside the regions coded for by the variable region exons and is thus expected to recognize all isoforms of human CD44 (Mackay et al. 1994).
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human T lymphocytes
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX30725 IHC-P Image
IHC-P analysis of human breast cancer biopsy tissue using GTX30725 CD44 antibody [F10-44-2].
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GTX30725 FACS Image
FACS analysis of human peripheral blood lymphocytes using GTX30725 CD44 antibody [F10-44-2].
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GTX30725 IHC-P Image
IHC-P analysis of human breast cancer biopsy tissue using GTX30725 CD44 antibody [F10-44-2].
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REFERENCE
REVIEW
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