GeneTex
United States (US)

CD44 antibody [F10-44-2]

GTX30725_2174_FACS_18121410_923.jpg
GTX30725_2178_IHC-P_18121410_828.jpg
GTX30725_2179_IHC-P_18121410_247.jpg

Cat No. GTX30725

Host Mouse
Clonality Monoclonal
Clone Name F10-44-2
Isotype IgG2a
Application ICC/IF, IHC-P, IHC-Fr, FACS
Reactivity Human, Cynomolgus monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
IHC-P 1/50-1/100
IHC-Fr Assay dependent
FACS 1/10-1/20

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes an epitope on human CD44 outside the regions coded for by the variable region exons and is thus expected to recognize all isoforms of human CD44 (Mackay et al. 1994).
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human T lymphocytes

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

LHR Antibody , HUTCH-I Antibody , CDW44 Antibody , ECMR-III Antibody , MDU2 Antibody , CSPG8 Antibody , Pgp1 Antibody , IN Antibody , MIC4 Antibody , MDU3 Antibody , MC56 Antibody , CD44 molecule (Indian blood group) Antibody , HCELL Antibody

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX30725_2174_FACS_18121410_923.jpg

GTX30725 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX30725 CD44 antibody [F10-44-2].

GTX30725_2178_IHC-P_18121410_828.jpg

GTX30725 IHC-P Image

IHC-P analysis of human breast cancer biopsy tissue using GTX30725 CD44 antibody [F10-44-2].

GTX30725_2179_IHC-P_18121410_247.jpg

GTX30725 IHC-P Image

IHC-P analysis of human breast cancer biopsy tissue using GTX30725 CD44 antibody [F10-44-2].

Package List Price ($)
$ 349