CD44 antibody [GT462]

Cat. No. GTX628895
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Cat. No.  GTX628895
KO/KD Orthogonal IP/MS Carrier-Free
Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895
GTX628895 WB Image
KO/KD

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:500.

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Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895
GTX628895 WB Image

Multiple bands were observed 130~180 kDa. It is possibly due to alternative splicing.
Reference: PMID: 20962267

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000.

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Anti-CD44 antibody [GT462] used in IHC (Paraffin sections) (IHC-P). GTX628895
GTX628895 IHC-P Image

CD44 antibody [GT462] detects CD44 protein at membrane on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
CD44 antibody [GT462] (GTX628895) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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Anti-CD44 antibody [GT462] used in Flow cytometry (FCM). GTX628895
GTX628895 FCM Image

CD44 antibody detects CD44 protein by flow cytometry analysis.
Sample: HL-60 cell fixed in 4% paraformaldehyde at 4oC for 5 min.
Black: Isotype control dilution: 1:100.
Green: CD44 antibody (GTX628895) dilution: 1:100.

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Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895
GTX628895 WB Image
Orthogonal

Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895
GTX628895 WB Image
KO/KD

The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.(br)Reference:PMID: 10050880

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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Anti-CD44 antibody [GT462] used in IHC (Paraffin sections) (IHC-P). GTX628895
GTX628895 IHC-P Image

CD44 antibody [GT462] detects CD44 protein at cell membrane by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human breast cancer.
CD44 stained by CD44 antibody [GT462] (GTX628895) diluted at 1:200.
Antigen Retrieval: EDTA buffer, 20 min

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Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895
Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895
Anti-CD44 antibody [GT462] used in IHC (Paraffin sections) (IHC-P). GTX628895
Anti-CD44 antibody [GT462] used in Flow cytometry (FCM). GTX628895
Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895
Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895
Anti-CD44 antibody [GT462] used in IHC (Paraffin sections) (IHC-P). GTX628895
This CD44 antibody [GT462] (GTX628895) is a mouse monoclonal antibody validated for detection of CD44 by WB, ICC/IF, IHC-P, and FCM. Reactivity has been shown in Human. Knockout/knockdown validation supports antibody specificity.
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    GT462
  • Isotype
    IgG2b
  • Applications
    WB ICC/IF IHC-P FCM
  • Reactivity
    Human
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