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CD44 antibody [GT462]

Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895
Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895
Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895
Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895
Anti-CD44 antibody [GT462] used in Flow cytometry (FACS). GTX628895
Anti-CD44 antibody [GT462] used in IHC (Paraffin sections) (IHC-P). GTX628895

Cat No. GTX628895

Host Mouse
Clonality Monoclonal
Clone Name GT462
Isotype IgG2b
Application WB, IHC-P, FACS, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

A431 , MDA-MB-231 , MDA-MB-231(24 μg/ml Tunicamycin treatment for 16 hr) , HeLa , HeLa(12 μg/ml Tunicamycin treatment for 48 hr) , HeLa(12 μg/ml Tunicamycin treatment for 48 hr and shCD44-transfected 293T)

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377425)

Predict Reactivity

Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD44. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD44 molecule (Indian blood group),CDW44,CSPG8,ECMR-III,HCELL,HUTCH-I,IN,LHR,MC56,MDU2,MDU3,MIC4,Pgp1

Cellular Localization

Membrane

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895

GTX628895 WB Image

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:500.

Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895

GTX628895 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000.
Multiple bands were observed 130~180 kDa. It is possibly due to alternative splicing.
Reference: PMID: 20962267

Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895

GTX628895 WB Image

Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD44 antibody [GT462] used in Western Blot (WB). GTX628895

GTX628895 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT462] (GTX628895) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The observed M.W. is different from the predicted size. It is possibly due to post-translational modifications.(br)Reference:PMID: 10050880

Anti-CD44 antibody [GT462] used in Flow cytometry (FACS). GTX628895

GTX628895 FACS Image

CD44 antibody detects CD44 protein by flow cytometry analysis.
Sample: HL-60 cell fixed in 4% paraformaldehyde at 4ºC for 5 min.
Black: Isotype control dilution: 1:100.
Green: CD44 antibody (GTX628895) dilution: 1:100.

Anti-CD44 antibody [GT462] used in IHC (Paraffin sections) (IHC-P). GTX628895

GTX628895 IHC-P Image

CD44 antibody [GT462] detects CD44 protein at membrane on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
CD44 antibody [GT462] (GTX628895) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169