GeneTex

  • Country / Location Selection

United States (US)

CD44 antibody [GT981]

KO/KD IP/MS
Anti-CD44 antibody [GT981] used in Western Blot (WB). GTX628472
Anti-CD44 antibody [GT981] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628472
Anti-CD44 antibody [GT981] used in IHC (Paraffin sections) (IHC-P). GTX628472
Anti-CD44 antibody [GT981] used in Western Blot (WB). GTX628472
Anti-CD44 antibody [GT981] used in Flow cytometry (FACS). GTX628472

Cat No. GTX628472

Reference 4
1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT981

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all CD44 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

HeLa , mouse leukocyte

Product Note

IP/MS validation was supported by references (PMID:30377421)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD44. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888037

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1

Cellular Localization

Membrane

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD44 antibody [GT981] used in Western Blot (WB). GTX628472

GTX628472 WB Image

KO/KD

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT981] (GTX628472) diluted at 1:500.

Anti-CD44 antibody [GT981] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628472

GTX628472 ICC/IF Image

CD44 antibody [GT981] detects CD44 protein at membrane by confocal immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: CD44 protein stained by CD44 antibody [GT981] (GTX628472) diluted at 1:500.
Blue: Hoechst 33342 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

Anti-CD44 antibody [GT981] used in IHC (Paraffin sections) (IHC-P). GTX628472

GTX628472 IHC-P Image

CD44 antibody [GT981] detects CD44 protein at cytosol and membrane on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
CD44 antibody [GT981] (GTX628472) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD44 antibody [GT981] used in Western Blot (WB). GTX628472

GTX628472 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [GT981] (GTX628472) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

Anti-CD44 antibody [GT981] used in Flow cytometry (FACS). GTX628472

GTX628472 FACS Image

CD44 antibody [GT981] (GTX628472) detects CD44 protein by flow cytometry analysis.
Sample: HeLa cell.
Black: Unlabelled sample was used as a control.
Red: CD44 antibody [GT981] (GTX628472) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

REFERENCE

Application Reference

An JH et al. BMC Vet Res 2018; 14 (1) : 354 Prostaglandin E2 secreted from feline adipose tissue-derived mesenchymal stem cells alleviate DSS-induced colitis by increasing regulatory T cells in mice.
Application : FACS
Reactivity : Mouse
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Huang TT et al. Oncotarget 2015; 6 (20) : 18012-26 The reciprocal regulation loop of Notch2 pathway and miR-23b in controlling gastric carcinogenesis.
Application : WB
Reactivity : Human
Yang J et al. J Res Med Sci 2014; 19 (2) : 112-6 Adipose-derived stem cells from the breast.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

CD44 antibody [GT981] Cat No. GTX628472

Rating
( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 1 users reviews)
Anti-CD44 antibody [GT981] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628472
Date : Anonymous submitted on 12-May-2016
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : RAW 264.7 cells
Amount used : 5x10^5
Fixation : 4% formaldehyde, 0.25Hr
Primary Antibody : 1:100, 4°C, 8Hr
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169