GeneTex

  • Country / Location Selection

United States (US)

CD44 antibody [HL1650]

Recombinant antibody KO/KD Comparison Orthogonal
Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231
Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231
Anti-CD44 antibody [HL1650] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637231
Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231
Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231
Anti-CD44 antibody [HL1650] used in IHC (Paraffin sections) (IHC-P). GTX637231
Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231
Anti-CD44 antibody [HL1650] used in IHC (Paraffin sections) (IHC-P). GTX637231
Anti-CD44 antibody [HL1650] used in IHC (Paraffin sections) (IHC-P). GTX637231

Cat. No. GTX637231

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1650

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all CD44 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Product Note

This antibody was raised against human CD44 Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CD44. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231

GTX637231 WB Image

KO/KD

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231

GTX637231 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-CD44 antibody [HL1650] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637231

GTX637231 ICC/IF Image

CD44 antibody [HL1650] detects CD44 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231

GTX637231 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231

GTX637231 WB Image

Orthogonal

Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD44 antibody [HL1650] used in IHC (Paraffin sections) (IHC-P). GTX637231

GTX637231 IHC-P Image

CD44 antibody [HL1650] detects CD44 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD44 antibody [HL1650] used in Western Blot (WB). GTX637231

GTX637231 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD44 antibody [HL1650] used in IHC (Paraffin sections) (IHC-P). GTX637231

GTX637231 IHC-P Image

CD44 antibody [HL1650] detects CD44 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog lung.
CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD44 antibody [HL1650] used in IHC (Paraffin sections) (IHC-P). GTX637231

GTX637231 IHC-P Image

CD44 antibody [HL1650] detects CD44 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat mammary gland.
CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for CD44 antibody [HL1650] (GTX637231). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for CD44 antibody [HL1650] (GTX637231). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169
Custom Conjugations