CD44 antibody [HL1650]

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.

CD44 antibody [HL1650] (GTX637231) detects CD44 protein by flow cytometry analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at 4ºC for 15 min and washed with staining buffer. The cells were following stained with CD44 antibody [HL1650] (GTX637231) diluted at 1:50 or a Rabbit IgG isotype control (GTX35035) at 4ºC for 1 hour.

Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CD44 antibody [HL1650] detects CD44 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CD44 antibody [HL1650] detects CD44 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat mammary gland.
CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CD44 antibody [HL1650] detects CD44 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog lung.
CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CD44 antibody [HL1650] (GTX637231) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CD44 antibody [HL1650] detects CD44 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: CD44 stained by CD44 antibody [HL1650] (GTX637231) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).