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CD44 antibody [Hermes-3]

Anti-CD44 antibody [Hermes-3] used in Flow cytometry (FACS). GTX03492
Anti-CD44 antibody [Hermes-3] used in IHC (Paraffin sections) (IHC-P). GTX03492
Anti-CD44 antibody [Hermes-3] used in IHC (Paraffin sections) (IHC-P). GTX03492
Anti-CD44 antibody [Hermes-3] used in Flow cytometry (FACS). GTX03492
Anti-CD44 antibody [Hermes-3] used in IHC (Paraffin sections) (IHC-P). GTX03492
Anti-CD44 antibody [Hermes-3] used in IHC (Paraffin sections) (IHC-P). GTX03492
Anti-CD44 antibody [Hermes-3] used in IHC (Paraffin sections) (IHC-P). GTX03492
Anti-CD44 antibody [Hermes-3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03492
Anti-CD44 antibody [Hermes-3] used in Western Blot (WB). GTX03492

Cat. No. GTX03492

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 Hermes-3

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($479)
See all CD44 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:10-1:50
IHC-P 1:25
FACS 1:50
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CD44 recombinant protein

Purification

Protein G purified followed by dialysis against PBS

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD44 antibody [Hermes-3] used in Flow cytometry (FACS). GTX03492

GTX03492 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX03492 CD44 antibody [Hermes-3]. Acquisition of >10, 000 events was performed.
Green : Primary antibody
Blue : Isotype control
Dilution : 1:50

Anti-CD44 antibody [Hermes-3] used in IHC (Paraffin sections) (IHC-P). GTX03492

GTX03492 IHC-P Image

IHC-P analysis of human skin tissue using GTX03492 CD44 antibody [Hermes-3].
Antigen retireval : Heat mediation with a citrate buffer (pH6)
Dilution : 1:25

Anti-CD44 antibody [Hermes-3] used in IHC (Paraffin sections) (IHC-P). GTX03492

GTX03492 IHC-P Image

IHC-P analysis of human esophagus carcinoma tissue using GTX03492 CD44 antibody [Hermes-3].

Anti-CD44 antibody [Hermes-3] used in Flow cytometry (FACS). GTX03492

GTX03492 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX03492 CD44 antibody [Hermes-3]. Acquisition of >10, 000 events was performed.
Green : Primary antibody
Blue : Isotype control
Dilution : 1:50

Anti-CD44 antibody [Hermes-3] used in IHC (Paraffin sections) (IHC-P). GTX03492

GTX03492 IHC-P Image

IHC-P analysis of human lung adenocarcinoma tissue using GTX03492 CD44 antibody [Hermes-3].
Antigen retireval : Heat mediation with a citrate buffer (pH6)
Dilution : 1:25

Anti-CD44 antibody [Hermes-3] used in IHC (Paraffin sections) (IHC-P). GTX03492

GTX03492 IHC-P Image

IHC-P analysis of human skin tissue using GTX03492 CD44 antibody [Hermes-3].
Antigen retireval : Heat mediation with a citrate buffer (pH6)
Dilution : 1:25

Anti-CD44 antibody [Hermes-3] used in IHC (Paraffin sections) (IHC-P). GTX03492

GTX03492 IHC-P Image

IHC-P analysis of human lung adenocarcinoma tissue using GTX03492 CD44 antibody [Hermes-3].
Antigen retireval : Heat mediation with a citrate buffer (pH6)
Dilution : 1:25

Anti-CD44 antibody [Hermes-3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03492

GTX03492 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX03492 CD44 antibody [Hermes-3].
Red : Primary antibody
Blue : DAPI
Dilution : 0.01 mg/ml

Anti-CD44 antibody [Hermes-3] used in Western Blot (WB). GTX03492

GTX03492 WB Image

WB analysis of various sample lysates using GTX03492 CD44 antibody [Hermes-3].
Lane 1 : HeLa whole cell lysate
Lane 2 : HUVEC whole cell lysate Lysate
Dilution : 1:2000
Loading : 20 μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 479