PRODUCT
Summary
The IM7 antibody recognizes CD44, a ubiquitously expressed cell surface receptor which is important for extracellular matrix organization, cell-cell and cell-matrix adhesion and migration. CD44 may be expressed in a number of different isoforms (splice variants) from the most typical or “standard” form, known as CD44s, to variants designated CD44v, e.g. CD44v1 or CD44v6. These receptors interact with several ligands, but most often associate with an extracellular matrix component hyaluronate, through which it mediates adhesion.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody reacts with all isoforms of mouse CD44.
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1
Purification
Purified by affinity chromatography
From tissue culture supernatant
Conjugation
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-CD44 antibody [IM7] (FITC) used in Flow cytometry (FACS). GTX80087](/templates/default/js/lightbox/images/loading.gif "Anti-CD44 antibody [IM7] (FITC) used in Flow cytometry (FACS). GTX80087")
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GTX80087 FACS Image
FACS analysis of C57Bl/6 splenocytes using GTX80087 CD44 antibody [IM7] (FITC).
Solid line : Primary antibody
Dashed line : FITC rat IgG2b isotype control
Antibody amount : 0.5 μg
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REFERENCE
REVIEW
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![Anti-CD44 antibody [IM7] (FITC) used in Flow cytometry (FACS). GTX80087](/upload/website/prouct_img/small/GTX80087/GTX80087_20201020_FACS_33_w_23061322_544.webp "Anti-CD44 antibody [IM7] (FITC) used in Flow cytometry (FACS). GTX80087")
![Anti-CD44 antibody [IM7] (FITC) used in Flow cytometry (FACS). GTX80087](/upload/website/prouct_img/normal/GTX80087/GTX80087_20201020_FACS_33_w_23061322_544.webp "Anti-CD44 antibody [IM7] (FITC) used in Flow cytometry (FACS). GTX80087")