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CD44 antibody [IM7]

Orthogonal
Anti-CD44 antibody [IM7] used in Western Blot (WB). GTX80086
Anti-CD44 antibody [IM7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80086
Anti-CD44 antibody [IM7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80086

Cat. No. GTX80086

Reference 4

Host

 Rat

Clonality

 Monoclonal

Clone Name

 IM7

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Human, Mouse, Cat, Dog, Horse
Package
100 μg ($399)
See all CD44 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Product Note

This antibody reacts with all isoforms of mouse CD44.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11163144

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD44 antibody [IM7] used in Western Blot (WB). GTX80086

GTX80086 WB Image

Orthogonal

WB analysis of HeLa cells (positive ; lane 2) and MOLT-4 (negative ; lane 1) cell lysates (non-reducing) using GTX80086 CD44 antibody [IM7].

Anti-CD44 antibody [IM7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80086

GTX80086 ICC/IF Image

The data was published in the 2022 in Bioengineered. PMID: 35109755

Anti-CD44 antibody [IM7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80086

GTX80086 ICC/IF Image

The data was published in the 2022 in Bioengineered. PMID: 35109755

REFERENCE

Application Reference

de S? Schiavo Matias G et al. Bioengineered 2022; 13 (2) : 3551-3565 In vivo biocompatibility analysis of the recellularized canine tracheal scaffolds with canine epithelial and endothelial progenitor cells.
Application : ICC/IF
Reactivity : Dog
Hendawy H et al. Animals (Basel) 2021; 11 (2) Tissue Harvesting Site Effect on the Canine Adipose Stromal Vascular Fraction Quantity and Quality.
Application : FACS
Reactivity : Dog
Chae HK et al. VETIMM 2017; S0165-2427 (16) : 30381-6 Immunomodulatory Effects of Soluble Factors Secreted by Feline Adipose Tissue-derived Mesenchymal Stem Cells.
Application : FACS
Reactivity : Cat
Lin CW et al. Acta Biomater 2016; cd44-specific nanoparticles for redox-triggered reactive oxygen species production and * release.
Application : ICC/IF, FACS
Reactivity : Human, Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399