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CD44 antibody [MEM-263] (PE)

Anti-CD44 antibody [MEM-263] (PE) used in Flow cytometry (FACS). GTX79959
Anti-CD44 antibody [MEM-263] (PE) used in Western Blot (WB). GTX79959

Cat. No. GTX79959

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MEM-263

Isotype

 IgG1

Application

 WB, FACS

Reactivity

 Human, Dog, Pig
Package
100 test ($399)
See all CD44 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
FACS 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Product Note

This antibody reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Monkey

Immunogen

COS-7 cells (African Green Monkey).

Purification

Purified by size-exclusion chromatography

Conjugation

Phycoerythrin (PE)

RRID

AB_11168954

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD44 antibody [MEM-263] (PE) used in Flow cytometry (FACS). GTX79959

GTX79959 FACS Image

Flow cytometry analysis of human peripheral blood (lymphocyte gate) using anti-CD44 (MEM-263) PE conjugate.

Anti-CD44 antibody [MEM-263] (PE) used in Western Blot (WB). GTX79959

GTX79959 WB Image

Western Blotting analysis (non-reducing conditions) of isolatedperipheral blood lymphocytes of various species using anti-CD44 (MEM-263). Lane 1: lysate of human PBL. Lane 2: lysate of canine PBL. Lane 3: lysate of porcine PBL

REFERENCE

Clone Reference

Nishimura M et al. Xenotransplantation 2019;:e12501 Development and characterization of novel clinical grade neonatal porcine bone marrow-derived mesenchymal stem cells.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399